Department of Biology of Bacteria, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland.
J Appl Microbiol. 2018 Nov;125(5):1253-1265. doi: 10.1111/jam.14026. Epub 2018 Jul 18.
Catheter-associated urinary tract infections (CAUTI) caused by Proteus mirabilis are very difficult to treat due to the ability of biofilm formation and drug resistance of these bacteria. The aim of this study was to assess the antibiofilm activity of phages and develop phage cocktail to combat biofilm of P. mirabilis strains.
Planktonic forms and biofilms of 50 tested uropathogenic P. mirabilis strains showed different sensitivity to 13 phages used. Phages 39APmC32, 65APm2833 and 72APm5211 presenting strong antibiofilm activity were selected as cocktail components. The antibiofilm activity of phage cocktails was similar or slightly higher than that of the most active phage. A three-phage cocktail inhibited biofilm formation and destroyed biofilms of the same number of strains or 2-3 strains more compared to a single phage. The components of the three-phage cocktail did not block each other's activity.
The potential of developed anti-P. mirabilis phage cocktail as an antibiofilm agent was proved.
In this study, three broad host range phages presenting strong anti-P. mirabilis biofilm activity were selected. Additionally, high stability of these viruses makes them a useful tool for controlling the biofilms.
由于生物膜形成能力和这些细菌的耐药性，奇异变形杆菌引起的导管相关尿路感染（CAUTI）非常难以治疗。本研究旨在评估噬菌体的抗生物膜活性，并开发噬菌体鸡尾酒来对抗奇异变形杆菌菌株的生物膜。
50 株测试的尿路致病性奇异变形杆菌浮游形式和生物膜对使用的 13 种噬菌体表现出不同的敏感性。噬菌体 39APmC32、65APm2833 和 72APm5211 表现出强烈的抗生物膜活性，被选为鸡尾酒成分。噬菌体鸡尾酒的抗生物膜活性与最活跃的噬菌体相似或略高。三噬菌体鸡尾酒抑制生物膜形成并破坏相同数量的菌株或比单个噬菌体多 2-3 株的生物膜。三种噬菌体鸡尾酒的成分不会相互阻断彼此的活性。
所开发的抗奇异变形杆菌噬菌体鸡尾酒作为抗生物膜剂的潜力已得到证明。
在这项研究中，选择了三种具有广泛宿主范围且对奇异变形杆菌具有强烈抗生物膜活性的噬菌体。此外，这些病毒的高稳定性使它们成为控制生物膜的有用工具。
