Yazdi Mahsa, Bouzari Majid, Ghaemi Ezzat Allah
Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran.
J Mol Microbiol Biotechnol. 2018;28(1):37-46. doi: 10.1159/000487137. Epub 2018 Apr 4.
Proteus mirabilis is one of the most common causes of urinary tract infection (UTI), particularly in patients undergoing long-term catheterization. Phage vB_PmiS-TH was isolated from wastewater with high lytic activity against P. mirabilis (TH) isolated from UTI. The phage had rapid adsorption, a large burst size (∼260 PFU per infected cell), and high stability at a wide range of temperatures and pH values. As analyzed by transmission electron microscopy, phage vB_PmiS-TH had an icosahedral head of ∼87 × 62 nm with a noncontractile tail about 137 nm in length and 11 nm in width. It belongs to the family Siphoviridae. Combination of the phage vB_PmiS-TH with ampicillin had a higher removal activity against planktonic cells of P. mirabilis (TH) than the phage or the antibiotic alone. Combination of the phage at a multiplicity of infection of 100 with a high dose of ampicillin (246 µg/mL) showed the highest biofilm removal activity after 24 h. This study demonstrates that using a combination of phage and antibiotic could be significantly more effective against planktonic and biofilm forms of P. mirabilis (TH).
奇异变形杆菌是尿路感染（UTI）最常见的病因之一，尤其是在长期接受导尿的患者中。噬菌体vB_PmiS-TH是从废水中分离出来的，对从UTI患者中分离出的奇异变形杆菌（TH）具有高裂解活性。该噬菌体具有快速吸附能力、较大的爆发量（每个感染细胞约260个噬菌斑形成单位），并且在广泛的温度和pH值范围内具有高稳定性。通过透射电子显微镜分析，噬菌体vB_PmiS-TH具有一个约87×62 nm的二十面体头部，带有一条长度约为137 nm、宽度为11 nm的非收缩性尾部。它属于长尾噬菌体科。噬菌体vB_PmiS-TH与氨苄西林联合使用对奇异变形杆菌（TH）的浮游细胞的清除活性高于单独使用噬菌体或抗生素。感染复数为100的噬菌体与高剂量氨苄西林（246 µg/mL）联合使用在24小时后显示出最高的生物膜清除活性。这项研究表明，噬菌体和抗生素联合使用对奇异变形杆菌（TH）的浮游和生物膜形式可能具有显著更高的效果。
