Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia, USA
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh.
J Clin Microbiol. 2018 Aug 27;56(9). doi: 10.1128/JCM.00702-18. Print 2018 Sep.
Two new monoclonal antibody-based, sandwich enzyme immunoassays (EIAs) for fecal antigen detection of or were evaluated using diarrheal stool specimens from a cohort of children in Bangladesh. These children routinely harbor multiple enteric pathogens, often at levels that make it difficult to assign diarrheal symptoms to a causative agent. A panel of 158 PCR-positive specimens with a broad range of / DNA cycle threshold ( ) values was used to assess the ability of the two tests to detect / antigen amounts that varied widely. A panel of 100 / PCR-negative specimens was used to verify that the assays correctly identified specimens as negative when the sample contained other enteric pathogens. Further analysis was conducted on a subset of 46 specimens that contained particularly substantial amounts of / ( of ≤19.7) that previous studies have ascribed as "diarrhea-associated." The Quik Chek rapid EIA and the Chek enzyme-linked immunosorbent assays (ELISAs) had a sensitivity of 95.7% for these specimens (specificities, 97% and 96%, respectively), supporting the usefulness of the new Chek and Quik Chek assays in symptomatic presentations, where is the likely etiology.
两种新的基于单克隆抗体的粪便抗原夹心酶免疫分析（EIA）用于检测或 ，使用孟加拉国一组儿童的腹泻粪便标本进行评估。这些儿童通常携带多种肠道病原体，其水平常常使得难以将腹泻症状归因于病原体。使用一组 158 个具有广泛 / DNA 循环阈值（）值的 PCR 阳性标本评估了两种检测方法检测/抗原量的能力，该抗原量差异很大。使用 100 个 / PCR 阴性标本来验证当样品中含有其他肠道病原体时，该测定正确地将样品鉴定为阴性。对包含特别大量/（≤19.7）的 46 个标本子集进行了进一步分析，先前的研究将这些/（≤19.7）鉴定为“腹泻相关”。对于这些标本，快速 EIA 和 Chek ELISA 的灵敏度分别为 95.7%（特异性分别为 97%和 96%），支持新的 Chek 和 Quik Chek 测定在症状出现时的有用性，因为是可能的病因。
