Federal University of Mato Grosso do Sul, Campo Grande, 79070-900 Mato Grosso do Sul, Brazil.
Universidade Católica Dom Bosco, Campo Grande, 79117-900 Mato Grosso do Sul, Brazil.
Nutrients. 2018 Jun 20;10(6):792. doi: 10.3390/nu10060792.
Polydextrose (PDX) ingestion may increase the intestinal absorption of iron. This study evaluated the effects of 7.5% polydextrose supplementation on markers of iron uptake, transport and storage in partially gastrectomized rats. Half of a batch of 40 male Wistar rats (250 g) underwent Billroth II partial gastrectomy with anterior truncal vagotomy (GXT), while the other half underwent sham gastrectomy (SHAM). At 7 postoperative days, the animals were subdivided into four groups ( = 10): Sham Control and GXT Control (no polydextrose); Sham PDX and GXT PDX (with 7.5% PDX). The animals were euthanized after 60 day of PDX treatment. Organ weight, cecal pH, the characterization and quantification of short-chain fatty acids (SCFA), hematological parameters, hepatic iron content and the expression of ferroportin (FPT) in the jejunum, cecum, colon and liver were evaluated. PDX caused changes in the cecum of the supplemented animals, where there was a decrease in pH, increase in cecal wall and marked production of SCFA, especially acetic and propionic acids ( < 0.05). Hepatic iron levels were lower in GXT animals. PDX increased hemoglobin (HGB) values by 29.2% and hematocrit (HCT) by 55.8% in the GXT PDX group compared to the GXT Control group. The GXT PDX group had lower hepatic FPT expression ( < 0.05). PDX led to increased SCFA concentration in the supplemented animals. Considering that SCFAs play a central role in the increasing nutrients uptake, this mechanism may be involved in altering the hematology profile observed in these animals but not enough to reverse iron deficiency anemia in post-gastrectomy rats.
聚右旋糖（PDX）的摄入可能会增加铁的肠道吸收。本研究评估了 7.5%聚右旋糖补充对部分胃切除大鼠铁吸收、转运和储存标志物的影响。一批 40 只雄性 Wistar 大鼠（250g）中的一半接受了 Billroth II 部分胃切除术和前干迷走神经切断术（GXT），另一半接受了假手术（SHAM）。术后 7 天，将动物分为四组（每组 10 只）：SHAM 对照组和 GXT 对照组（无聚右旋糖）；SHAM PDX 组和 GXT PDX 组（7.5% PDX）。PDX 治疗 60 天后，动物被安乐死。评估器官重量、盲肠 pH 值、短链脂肪酸（SCFA）的特征和定量、血液学参数、肝脏铁含量以及空肠、盲肠、结肠和肝脏中铁蛋白（FPT）的表达。PDX 导致补充动物盲肠发生变化，pH 值降低，盲肠壁增厚，SCFA 大量产生，特别是乙酸和丙酸（<0.05）。GXT 动物的肝铁水平较低。与 GXT 对照组相比，GXT PDX 组血红蛋白（HGB）值增加了 29.2%，血细胞比容（HCT）增加了 55.8%。GXT PDX 组肝 FPT 表达降低（<0.05）。PDX 导致补充动物的 SCFA 浓度增加。考虑到 SCFAs 在增加营养物质吸收中起着核心作用，这种机制可能参与改变这些动物观察到的血液学特征，但不足以逆转胃切除术后大鼠的缺铁性贫血。
