Wang Jinxing, Lin Jing, Kan Jialiang, Wang Hong, Li Xiaogang, Yang Qingsong, Li Hui, Chang Youhong
Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Pomology, Jiangsu Academy of Agricultural Sciences, Nanjing 200014, China.
Genes (Basel). 2018 Jun 20;9(6):311. doi: 10.3390/genes9060311.
Increasing evidence shows that long noncoding RNAs (lncRNAs) play important roles in developmental regulation and many other biological processes in plants. However, identification of lncRNAs in is limited compared with studies of functional gene expression. Using high-throughput sequencing technology, the transcriptome of . under drought stress was analyzed to identify lncRNAs. A total of 14,478 lncRNAs were identified, of which 251 were found to be drought-responsive. The putative target genes of these differentially expressed lncRNAs were significantly enriched in metabolic processes, organic substance metabolic processes, macromolecule metabolic processes, and heterocyclic compound binding. Real-time quantitative polymerase chain reaction validation suggested that the results of the RNA sequencing data analysis were reliable. This study will provide genetic resources for pear breeding and provide reference to other pomological studies.
越来越多的证据表明，长链非编码RNA（lncRNAs）在植物的发育调控和许多其他生物学过程中发挥着重要作用。然而，与功能基因表达的研究相比，梨中lncRNAs的鉴定较为有限。利用高通量测序技术，对干旱胁迫下梨的转录组进行分析以鉴定lncRNAs。共鉴定出14478个lncRNAs，其中251个被发现对干旱有响应。这些差异表达lncRNAs的推定靶基因在代谢过程、有机物质代谢过程、大分子代谢过程和杂环化合物结合中显著富集。实时定量聚合酶链反应验证表明RNA测序数据分析结果可靠。本研究将为梨育种提供遗传资源，并为其他果树学研究提供参考。
