Institute of Pomology, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, China.
PLoS One. 2018 Jul 17;13(7):e0200692. doi: 10.1371/journal.pone.0200692. eCollection 2018.
Circular RNAs (circRNAs) play important roles in miRNA function and transcriptional control. However, little is known regarding circRNAs in the pear. In this study, we identified circRNAs using deep sequencing and analyzed their expression under drought stress. We identified 899 circRNAs in total, among which 33 (23 upregulated, 10 downregulated) were shown to be dehydration-responsive. We performed GO and KEGG enrichment analysis to predict the functions of differentially expressed circRNAs. 309 circRNAs were predicted to act as sponges for 180 miRNAs. A circRNA-miRNA co-expression network was constructed based on correlation analysis between the differentially expressed circRNAs and their miRNA binding sites. Our study will provide a rich genetic resource for the discovery of genes related to drought stress, and can readily be applied to other fruit trees.
环状 RNAs (circRNAs) 在 miRNA 功能和转录调控中发挥重要作用。然而，关于梨中的 circRNAs 知之甚少。在这项研究中，我们使用深度测序鉴定了 circRNAs，并分析了它们在干旱胁迫下的表达情况。我们总共鉴定了 899 个 circRNAs，其中 33 个（23 个上调，10 个下调）被证明是对脱水有反应的。我们进行了 GO 和 KEGG 富集分析，以预测差异表达 circRNAs 的功能。309 个 circRNAs 被预测为 180 个 miRNA 的海绵。根据差异表达 circRNAs 与其 miRNA 结合位点之间的相关性分析，构建了一个 circRNA-miRNA 共表达网络。我们的研究将为发现与干旱胁迫相关的基因提供丰富的遗传资源，并且可以很容易地应用于其他果树。
