金丝桃素对大肠杆菌的光动力灭活作用。
Effect of photodynamic inactivation of Escherichia coli by hypericin.
机构信息
College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.
School of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong, China.
出版信息
World J Microbiol Biotechnol. 2018 Jun 20;34(7):100. doi: 10.1007/s11274-018-2464-1.
The present study has focused on the effects of hypericin (Hyp) based photodynamic inactivation (PDI) of Escherichia coli (E. coli). To evaluate the efficiency of Hyp based PDI of E. coli, single factor experiments and response surface optimization experiment were conducted to obtain the optimum parameter values (36 µM Hyp, 5.9 J cm light dose: 16.4 mW cm, 60 W, 260 s, 590 nm and 68 min incubation time) and finally achieved a 4.1 log CFU mL decrease of E. coli. Cell-Hyp interaction and intracellular reactive oxygen species (ROS) level were detected by fluorescence spectrometric photometer. Data indicated that Hyp possessed a strong ability to bind with cells. In addition, a significant increase was observed in intracellular ROS level after Hyp-based photosensitization treatment. Therefore, Hyp-based photosensitization seems to be a promising method to efficiently inactivate E. coli. It is expected to be a safe, efficient, low cost and practical method which can be applied in the field of food safety.
本研究集中于金丝桃素（Hyp）的光动力灭活（PDI）对大肠杆菌（E. coli）的影响。为了评估 Hyp 基 PDI 对 E. coli 的效率，进行了单因素实验和响应面优化实验，以获得最佳参数值（36 μM Hyp、5.9 J cm 光剂量：16.4 mW cm、60 W、260 s、590 nm 和 68 min 孵育时间），最终使 E. coli 的减少量达到 4.1 log CFU mL。通过荧光分光光度计检测细胞-Hyp 相互作用和细胞内活性氧（ROS）水平。数据表明 Hyp 具有与细胞结合的强大能力。此外，在 Hyp 基光致敏处理后，细胞内 ROS 水平显著升高。因此，Hyp 基光致敏似乎是一种有效灭活 E. coli 的有前途的方法。它有望成为一种安全、高效、低成本且实用的方法，可应用于食品安全领域。
Zotero 插件