Matsubara Teruhiko, Sato Toshinori, Taki Takao
Department of Biosciences and Informatics, Keio University, Kouhoku, Yokohama, Japan.
AGT & T Co. Ltd., Oujinncho, Tokushima, Japan.
Methods Mol Biol. 2018;1804:437-447. doi: 10.1007/978-1-4939-8552-4_23.
"Glycoreplica peptides" are prepared using a phage display peptide library and monoclonal antibodies that recognize the carbohydrate epitopes of glycoconjugate antigens. The peptides obtained not only mimic the shapes of original glycoconjugate antigens but also have some of their functions. We herein describe how to identify the amino acid alignments of glycoreplica peptides using phage display selection against carbohydrate-binding proteins. Target-specific peptides and proteins may be selected from the large repertory of a peptide/protein library using phage display technology. Glycoreplica peptides have the potential to become alternatives to carbohydrate ligands such as mimotopes for vaccinations and carbohydrate-derived drugs for carbohydrate-related diseases.
“糖复现肽”是利用噬菌体展示肽库和识别糖缀合物抗原碳水化合物表位的单克隆抗体制备而成。所获得的肽不仅模拟了原始糖缀合物抗原的形状，还具备其一些功能。我们在此描述如何通过针对碳水化合物结合蛋白的噬菌体展示筛选来鉴定糖复现肽的氨基酸序列。利用噬菌体展示技术可从肽/蛋白质库的大量储备中筛选出靶标特异性肽和蛋白质。糖复现肽有潜力成为碳水化合物配体的替代品，如用于疫苗接种的模拟表位以及用于治疗碳水化合物相关疾病的碳水化合物衍生药物。
