Bertucci Alessandro, Silvestrini Simone, Corradini Roberto, De Cola Luisa
Institut de Science et d'Ingénierie Supramoléculaires (ISIS) Université de Strasbourg, Strasbourg, France.
Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy.
Methods Mol Biol. 2018;1811:65-77. doi: 10.1007/978-1-4939-8582-1_5.
Peptide Nucleic Acids (PNAs) are oligonucleotide mimics that can be used as drugs as they can interact with DNA and RNA targets in organisms. Loading PNAs into inorganic nanocarriers can improve their cellular uptake and co-delivering them together with drugs can improve the therapy efficacy by synergic effects. Furthermore, the functionalization of the carriers with labels allows theranostics, and the possibility to monitor the efficacy of the therapy in real time. The present protocol describes the synthesis of Zeolites-L nanocrystals and mesoporous silica nanoparticles and their loading with cationic PNAs and other smaller molecular weight payloads towards theranostics applications.
肽核酸（PNA）是一种寡核苷酸类似物，可作为药物，因为它们能够与生物体内的DNA和RNA靶点相互作用。将PNA加载到无机纳米载体中可以提高其细胞摄取率，并且将它们与药物共同递送可以通过协同效应提高治疗效果。此外，用标记物对载体进行功能化可实现治疗诊断，并有实时监测治疗效果的可能性。本方案描述了沸石-L纳米晶体和介孔二氧化硅纳米颗粒的合成，以及它们负载阳离子PNA和其他较小分子量负载物用于治疗诊断应用的过程。
