Idili Andrea, Ricci Francesco
Department of Chemistry, University of Rome Tor Vergata, Rome, Italy.
Methods Mol Biol. 2018;1811:79-100. doi: 10.1007/978-1-4939-8582-1_6.
Triplex DNA is becoming a very useful domain to design pH-triggered DNA nanoswitches and nanodevices. The high versatility and programmability of triplex DNA interactions allows the integration of pH-controllable modules into DNA-based reactions and self-assembly processes. Here, we describe the procedure to characterize DNA-based triplex nanoswitches and more in general pH-triggered structure-switching mechanisms. Procedures to characterize pH-triggered DNA nanodevices will be useful for many applications in the field of biosensing, drug delivery systems and smart nanomaterials.
三链DNA正成为设计pH触发的DNA纳米开关和纳米器件的一个非常有用的领域。三链DNA相互作用的高度通用性和可编程性使得将pH可控模块整合到基于DNA的反应和自组装过程中成为可能。在这里，我们描述了表征基于DNA的三链纳米开关以及更一般的pH触发结构转换机制的程序。表征pH触发的DNA纳米器件的程序将对生物传感、药物递送系统和智能纳米材料领域的许多应用有用。
