Wang Zhi-Wang, Fu Xiao-Yan, Cheng Xiao-Li, Bao Xiao-Ying, Liu Xue-Feng, Duan Hai-Jing
Gansu University of Chinese Medicine, Lanzhou 730000.
Affiliated Hospital of Gansu University of Chinese Medicine, Lanzhou 730000, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2018 Feb 8;34(2):169-172. doi: 10.12047/j.cjap.5593.2018.041.
To observe the preventive and therapeutic action of Yuyin Ruangan Granule (YRG, Traditional Chinese Medicine) in hepatic fibrosis rats model and its effect on transforming growth factor-β1 (TGF-β1) expression.
The Wistar rats were randomly divided into 6 group (=10), and the model of hepatic fibrosis rats was established by subcutaneous injected with carbon tetrachloride (CCL4), fed on high-fat diet and 20% ethanol for 6 weeks, to survey the effect and mechanism of YRG preventing hypatic fibrosis by detecting liver function (the activity of alanine aminotransferase(ALT), aspartate aminotransferase(AST), etc.) of liver fibrosis rats, liver fibrosis indicators (hyaluronic acid, Ⅲ procollagen, type IV collagen, laminin and hepatic pathology, etc.), and TGF-β1 expression in liver tissue after 6 weeks treated with YRG through intragastric administration (q. d.).
At the 7 week, fibrotic lesions appears distinctly in liver tissue of model group compared with control group (<0.01), YRG of 6.2~28.8 g/kg could significantly decrease hepatic index, ALT and AST activities, content of hyaluronic acid(HA), Ⅲ procollagen (PCⅢ), type Ⅳ collagen(C-Ⅳ), laminin (LN) in serum, relieve liver fibrosis pathological changes and inhibit TGF-β1 expression in fibrotic liver tissue (<0.05, <0.01).
YRG has significantly preventive effects on liver fibrosis rats model, and it may be one of its mechanisms to inhibit expression of TGF-β1.
观察中药育阴软肝颗粒（YRG）对肝纤维化大鼠模型的防治作用及其对转化生长因子-β1（TGF-β1）表达的影响。
将Wistar大鼠随机分为6组（每组10只），采用皮下注射四氯化碳（CCL4）、高脂饮食并给予20%乙醇的方法建立肝纤维化大鼠模型，连续6周，通过检测肝纤维化大鼠的肝功能（谷丙转氨酶（ALT）、谷草转氨酶（AST）等活性）、肝纤维化指标（透明质酸、Ⅲ型前胶原、Ⅳ型胶原、层粘连蛋白及肝脏病理等）以及YRG灌胃给药（每日1次）6周后肝组织中TGF-β1的表达，探讨YRG防治肝纤维化的作用及机制。
第7周时，模型组肝组织纤维化病变较对照组明显（P<0.01），6.2~28.8 g/kg的YRG可显著降低肝指数、ALT和AST活性、血清透明质酸（HA）、Ⅲ型前胶原（PCⅢ）、Ⅳ型胶原（C-Ⅳ）、层粘连蛋白（LN）含量，减轻肝纤维化病理改变，抑制纤维化肝组织中TGF-β1的表达（P<0.05，P<0.01）。
YRG对肝纤维化大鼠模型有显著的防治作用，抑制TGF-β1表达可能是其作用机制之一。
