Ding Mao-Peng, Wei Ling-Xia, Wang Zhi-Wang, Cheng Xiao-Li, Pang Ya-Rong
Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2021 Sep;37(5):463-467. doi: 10.12047/j.cjap.6098.2021.045.
To investigate the therapeutic effects of Biejia Yugan Granule on hepatic fibrosis caused by compound factors in rats and its effect on TGF-β1/Smads signaling pathway. SD rats were randomly divided into blank control group, model control group, colchicine group, Biejia Yugan Granule low, medium and high dose (1.85, 3.70, 7.40 g/kg) groups (= 8 in each group). The rat model of hepatic fibrosis was established by treating with 5% alcohol 15 ml/kg (ig) everyday and injecting with 40% carbon tetrachloride (sc) twice a week for 42 days. The effects of Biejia Yugan Granule on liver function, liver index and water content, serum hepatic fibrosis related indicators, key proteins and gene expression of TGF-β1/Smads signaling pathway in rats were observed. Biejia Yugan Granule at the doses of 1.85, 3.70 and 7.40 g/kg could decrease the serum levels of ALT, AST, ALP and HA, PCⅢ, C-Ⅳ, LN significantly, reduce the water content of liver tissue leads to the decrease of liver index, regulate the liver tissue TGF-β1, Smad3 mRNA and Smad7 mRNA expressions. Biejia Yugan Granule has obvious effects of reducing enzyme and protecting liver and inhibiting hepatic fibrosis, and inhibiting TGF-β1/Smads signaling pathway is one of its mechanisms of anti-hepatic fibrosis.
探讨鳖甲愈肝颗粒对复合因素所致大鼠肝纤维化的治疗作用及其对转化生长因子-β1（TGF-β1）/Smads信号通路的影响。将SD大鼠随机分为空白对照组、模型对照组、秋水仙碱组、鳖甲愈肝颗粒低、中、高剂量（1.85、3.70、7.40 g/kg）组（每组n = 8）。采用每日以5%乙醇15 ml/kg灌胃，每周2次皮下注射40%四氯化碳的方法，连续造模42天，建立大鼠肝纤维化模型。观察鳖甲愈肝颗粒对大鼠肝功能、肝指数、肝组织含水量、血清肝纤维化相关指标、TGF-β1/Smads信号通路关键蛋白及基因表达的影响。鳖甲愈肝颗粒1.85、3.70、7.40 g/kg剂量组能显著降低血清谷丙转氨酶（ALT）、谷草转氨酶（AST）、碱性磷酸酶（ALP）及透明质酸（HA）、Ⅲ型前胶原（PCⅢ）、Ⅳ型胶原（C-Ⅳ）、层粘连蛋白（LN）水平，降低肝组织含水量，使肝指数下降，调节肝组织TGF-β1、Smad3 mRNA及Smad7 mRNA表达。鳖甲愈肝颗粒具有明显的降酶保肝及抗肝纤维化作用，抑制TGF-β1/Smads信号通路是其抗肝纤维化机制之一。
