Li Rui-Qing, Wan Ming-Yue, Shi Jing, Wang Hui-Ling, Liu Fei-Lai, Liu Cheng-Mei, Huang Jin, Liu Ren-Chao, Ma Le, Feng Xiao-Dong
Rehabilitation Center, First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
Major in Rehabilitation Medicine and Physiotherapy, Henan University of Traditional Chinese Medicine, Zhengzhou, Henan Province, China.
Neural Regen Res. 2018 Jun;13(6):1013-1018. doi: 10.4103/1673-5374.233444.
Catgut implantation at acupoints has been shown to alleviate spasticity after stroke in rats. However, the underlying mechanisms are poorly understood. In this study, we used the rat middle cerebral artery occlusion model of stroke. Three days after surgery, absorbable surgical catgut sutures were implanted at Dazhui (GV14), Jizhong (GV6), Houhui, Guanyuan (CV4) and Zhongwan (CV12). The Zea Longa score was used to assess neurological function. The Modified Ashworth Scale was used to evaluate muscle tension. The 2,3,5-triphenyl-tetrazolium chloride assay was used to measure infarct volume. Immunohistochemical staining was performed for glutamate aspartate transporter (GLAST) and glial glutamate transporter-1 (GLT-1) expression. Western blot assay was used to analyze the expression of GLAST and GLT-1. Reverse transcription and polymerase chain reaction were carried out to assess the expression of GLAST and GLT-1 mRNAs. After catgut implantation at the acupoints, neurological function was substantially improved, muscle tension was decreased, and infarct volume was reduced in rats with spasticity after stroke. Furthermore, the expression of GLAST and GLT-1 mRNAs was increased on the injured (left) side. Our findings demonstrate that catgut implantation at acupoints alleviates spasticity after stroke, likely by increasing the expression of GLAST and GLT-1.
穴位埋线已被证明可减轻大鼠中风后的痉挛。然而，其潜在机制尚不清楚。在本研究中，我们使用了大鼠大脑中动脉闭塞性中风模型。手术后三天，将可吸收手术缝线埋入大椎（GV14）、脊中（GV6）、后会、关元（CV4）和中脘（CV12）。采用Zea Longa评分评估神经功能。采用改良Ashworth量表评估肌张力。采用2,3,5-三苯基氯化四氮唑试验测量梗死体积。对谷氨酸天冬氨酸转运体（GLAST）和胶质谷氨酸转运体-1（GLT-1）表达进行免疫组织化学染色。采用蛋白质免疫印迹法分析GLAST和GLT-1的表达。进行逆转录和聚合酶链反应以评估GLAST和GLT-1 mRNA的表达。穴位埋线后，中风后痉挛大鼠的神经功能得到显著改善，肌张力降低，梗死体积减小。此外，受伤（左侧）侧GLAST和GLT-1 mRNA的表达增加。我们的研究结果表明，穴位埋线可减轻中风后的痉挛，可能是通过增加GLAST和GLT-1的表达来实现的。
