通过噬菌体展示生成基于纤连蛋白结构域3的亲和试剂。
Generating FN3-Based Affinity Reagents Through Phage Display.
作者信息
Gorman Kevin, McGinnis Jennifer, Kay Brian
机构信息
Department of Biological Sciences, University of Illinois at Chicago, Chicago, Illinois.
出版信息
Curr Protoc Chem Biol. 2018 Jun;10(2):e39. doi: 10.1002/cpch.39. Epub 2018 Jun 7.
Abstract
Antibodies are useful tools for detecting individual proteins in complex samples and for learning about their location, amount, binding partners, and function in cells. Unfortunately, generating antibodies is time consuming and laborious, and their affinity and/or specificity is often limited. This protocol offers a fast and inexpensive alternative to generate antibody surrogates through phage display of a library of fibronectin type III (FN3) monobody variants and affinity selection for binders. © 2018 by John Wiley & Sons, Inc.
摘要
抗体是用于检测复杂样本中单个蛋白质以及了解其在细胞中的位置、数量、结合伴侣和功能的有用工具。不幸的是，生成抗体既耗时又费力，而且它们的亲和力和/或特异性往往有限。本方案提供了一种快速且廉价的替代方法，可通过对III型纤连蛋白（FN3）单域抗体变体文库进行噬菌体展示以及对结合物进行亲和选择来生成抗体替代物。© 2018约翰威立父子公司版权所有。
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