Angiolillo P J, Donoso L A, Folberg R, Augsburger J J, Wax M
Cancer Biochem Biophys. 1985 Jun;8(1):61-5.
Electron spin resonance (ESR) and high pressure liquid chromatography (HPLC) were utilized to characterize the physical and biochemical characteristics of melanin in choroidal melanoma. ESR free radical signals indicative of eumelanin could be elicited from formalin-fixed paraffin-embedded tissues. Zinc ions (50 mM) increased the number of melanin-free radicals resulting in greater ESR sensitivity. Pyrole 2,3,6 tricarboxylic acid (PTCA) and amino hydroxy phenylalanine (AHP) were identified by HPLC after permanganate oxidation and hydroiodic acid hydrolysis, respectively, of a choroidal melanoma obtained at enucleation. The results indicate eumelanin is the primary melanin type in posterior choroidal melanomas. The feasibility of these techniques in the detection of metastatic disease from ocular melanomas is discussed.
利用电子自旋共振（ESR）和高压液相色谱（HPLC）对脉络膜黑色素瘤中黑色素的物理和生化特性进行表征。福尔马林固定石蜡包埋组织可引发指示真黑素的ESR自由基信号。锌离子（50 mM）增加了黑色素自由基的数量，从而提高了ESR的灵敏度。分别通过对摘除眼球时获得的脉络膜黑色素瘤进行高锰酸盐氧化和氢碘酸酸解后，用HPLC鉴定出吡咯2,3,6-三羧酸（PTCA）和氨基羟基苯丙氨酸（AHP）。结果表明真黑素是脉络膜后黑色素瘤中的主要黑色素类型。讨论了这些技术在检测眼黑色素瘤转移性疾病中的可行性。
