Yang Dong, Zhang Weiyuan, Liu Yang, Liang Junqing, Zhang Tongqing, Bai Yunbo, Hao Wenjing, Ma Kexin, Lu Danni, Chen Jing
Department of Gynaecology, Beijing Obstetrics and Gynaecology Hospital, Capital Medical University, Beijing 100026, P.R. China.
Department of Gynaecology, Peking University People's Hospital, Beijing 100044, P.R. China.
Oncol Lett. 2018 Jun;15(6):9633-9640. doi: 10.3892/ol.2018.8567. Epub 2018 Apr 25.
Single-cell sequencing technology is a promising systematic and comprehensive approach to delineate clonal associations between cells. The present study collected 13 and 12 cervical cells from fresh tumour tissue prior to and following radiotherapy, respectively, from a 46-year-old female patient with exogenous-type cervical carcinoma. Next, single-cell whole-genome sequencing analysis was performed on each cell. Examination revealed that normal cells could be clearly distinguished from tumour cells among the 25 cells. Tumour cells prior to and following radiotherapy almost represented two independent clones, with the main subpopulation prior to radiotherapy being killed and the minor subpopulation prior to radiotherapy becoming the main subpopulation following radiotherapy. A human papillomavirus (HPV) integration site was detected in POU class 5 homeobox 1B () in tumour cells following radiotherapy, which has been reported to be a frequent HPV integration site in cervical carcinoma. These results indicate that tumour cells with HPV integration in survive radiotherapy, and that tumour cells prior to and following radiotherapy exhibit distinct characteristics.
单细胞测序技术是一种很有前景的系统且全面的方法，用于描绘细胞间的克隆关联。本研究从一名46岁的外生型宫颈癌女性患者的新鲜肿瘤组织中，分别在放疗前和放疗后收集了13个和12个宫颈细胞。接下来，对每个细胞进行了单细胞全基因组测序分析。检查发现，在这25个细胞中正常细胞可与肿瘤细胞清晰区分。放疗前和放疗后的肿瘤细胞几乎代表两个独立的克隆，放疗前的主要亚群被杀死，放疗前的次要亚群在放疗后成为主要亚群。在放疗后的肿瘤细胞中，在POU第5类同源盒1B（）中检测到一个人乳头瘤病毒（HPV）整合位点，据报道该位点是宫颈癌中常见的HPV整合位点。这些结果表明，具有HPV整合的肿瘤细胞在放疗后存活，且放疗前和放疗后的肿瘤细胞表现出不同的特征。
