Chen Binghai, Jiao Zhimin, Yin Xifeng, Qian Zhounan, Gu Jie, Sun Hao
Department of Urology, The Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212000, P.R. China.
Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212000, P.R. China.
Oncol Lett. 2018 Jul;16(1):83-90. doi: 10.3892/ol.2018.8665. Epub 2018 May 8.
Renal cell carcinoma (RCC) is a common form of cancer of the urinary tract. The present study aimed to identify driver genes in RCC using a bioinformatics approach. GSE53757 and GSE40435 microarray data were analyzed, and differentially expressed genes were filtered prior to gene ontology (GO) and pathway analysis. A protein-protein interaction (PPI) network was established. Overall survival and recurrence were investigated and based on data presented in cBioPortal. The COPS7B gene within the PPI network was selected for further study . The present study identified 174 and 149 genes possessing a significant signal to noise ratio in GSE53757 and GSE40435, respectively. In total, 53 of these genes were selected based upon inclusion in both datasets. GO analysis indicated that PRKCDBP, EHD2, KCNJ10, ATP1A1, KCNJ1 and EHD2 may be involved in various biological processes. Furthermore, ALDH6A1, LDHA, SUCLG1 and ABAT may be involved in the propanoate metabolism pathway. A network consisting of 106 genes, and one typical cluster were constructed. In addition, COPS7B was selected, as it was associated with decreased overall survival and increased recurrence rates, in order to elucidate its function in RCC. Furthermore, upregulation of COPS7B was demonstrated to be predictive of advanced stage disease and metastasis of RCC. Finally, COPS7B-knockdown inhibited RCC cell proliferation and invasion ability. Collectively, these results provided novel insights into COPS7B function, indicating that COPS7B may serve as a prognostic marker and therapeutic target in RCC.
肾细胞癌（RCC）是泌尿系统癌症的一种常见形式。本研究旨在采用生物信息学方法鉴定肾细胞癌中的驱动基因。分析了GSE53757和GSE40435微阵列数据，并在进行基因本体（GO）和通路分析之前对差异表达基因进行了筛选。建立了蛋白质-蛋白质相互作用（PPI）网络。根据cBioPortal中呈现的数据对总生存期和复发情况进行了研究。选择PPI网络中的COPS7B基因进行进一步研究。本研究分别在GSE53757和GSE40435中鉴定出174个和149个具有显著信噪比的基因。基于两个数据集中均包含这些基因，总共选择了其中53个基因。GO分析表明，PRKCDBP、EHD2、KCNJ10、ATP1A1、KCNJ1和EHD2可能参与各种生物学过程。此外，ALDH6A1、LDHA、SUCLG1和ABAT可能参与丙酸代谢途径。构建了一个由106个基因组成的网络和一个典型聚类。此外，选择COPS7B是因为它与总生存期降低和复发率增加相关，以阐明其在肾细胞癌中的功能。此外，COPS7B的上调被证明可预测肾细胞癌的晚期疾病和转移。最后，COPS7B基因敲低抑制了肾细胞癌细胞的增殖和侵袭能力。总的来说，这些结果为COPS7B的功能提供了新的见解，表明COPS7B可能作为肾细胞癌的预后标志物和治疗靶点。
