Ding Guowei, Liu Yongchao, Liang Chunmei
Department of Radiotherapy, Tianjin Fifth Central Hospital, Tianjin 300450, P.R. China.
Oncol Lett. 2018 Jul;16(1):219-224. doi: 10.3892/ol.2018.8622. Epub 2018 May 2.
The present study intended to investigate efficacy of radiotherapy in the treatment of intermediate and advanced stage lung cancer and the effects on serum vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9). Serum levels of VEGF and MMP-9 of 77 patients with intermediate or advanced lung cancer were detected before and after the treatment. At the same time, 19 healthy people were selected as the control group. Gelatin zymography was applied to measure the activity of serum MMP-9, ELISA was performed to detect the VEGF and MMP-9 levels in the peripheral blood and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to measure the messenger RNA (mRNA) levels of VEGF and MMP-9 in serum. Results indicated that the overall response rate of radiotherapy on intermediate and advanced lung cancer reached 70.1%. Levels of serum VEGF and MMP-9 in the effective treatment group were significantly lower than those before the treatment (P<0.05). Furthermore, expression levels of VEGF and MMP-9 in the effective radiotherapy group were significantly lower than those in ineffective group (P<0.05), MMP-9 activity before treatment was significantly higher than that after treatment (P<0.05) and expression levels of VEGF and MMP-9 mRNA before treatment were significantly elevated compared with those after treatment (P<0.05). The results suggested that the expression levels of serum VEGF and MMP-9 may be useful indicators for the evaluation of the efficacy of radiotherapy in the treatment of intermediate and advanced lung cancer.
本研究旨在探讨放射治疗在中晚期肺癌治疗中的疗效以及对血清血管内皮生长因子（VEGF）和基质金属蛋白酶-9（MMP-9）的影响。检测77例中晚期肺癌患者治疗前后血清VEGF和MMP-9水平。同时，选取19名健康人作为对照组。采用明胶酶谱法检测血清MMP-9活性，采用酶联免疫吸附测定法（ELISA）检测外周血中VEGF和MMP-9水平，采用逆转录定量聚合酶链反应（RT-qPCR）检测血清中VEGF和MMP-9的信使核糖核酸（mRNA）水平。结果显示，放射治疗对中晚期肺癌的总有效率达到70.1%。有效治疗组血清VEGF和MMP-9水平显著低于治疗前（P<0.05）。此外，有效放疗组VEGF和MMP-9的表达水平显著低于无效组（P<0.05），治疗前MMP-9活性显著高于治疗后（P<0.05），治疗前VEGF和MMP-9 mRNA的表达水平显著高于治疗后（P<0.05）。结果提示，血清VEGF和MMP-9的表达水平可能是评估放射治疗中晚期肺癌疗效的有用指标。
