巨细胞病毒总抗体酶免疫测定法评估。一种用于检测巨细胞病毒抗体的酶免疫测定法。

Evaluation of CMV total antibody EIA. An enzyme immunoassay for detection of antibodies to cytomegalovirus.

作者信息

Chairez R, Cesario A J, Barrett J E, Schleicher J B

出版信息

Diagn Microbiol Infect Dis. 1985 Sep;3(5):403-10. doi: 10.1016/0732-8893(85)90079-3.

Abstract

The performance of an indirect enzyme-immunoassay (CMV Total Antibody EIA, Abbott Laboratories) for the detection of the presence or absence of antibodies to cytomegalovirus (CMV) was evaluated by investigators at five clinical sites. The interassay and intraassay coefficient of variation data showed that the EIA could be performed in a highly reproducible manner at all sites. The investigators tested a total of 2314 clinical samples for antibody to CMV by the CMV Total Antibody EIA and by an indirect hemagglutination assay (IHA). The overall results demonstrated that compared with IHA the sensitivity of the EIA was 97.9% (1096/1120) and the specificity was 93.6% (1096/1171). The clinical samples tested included sera, plasma, and whole blood. These results show a good agreement between the CMV Total Antibody EIA and the IHA. This EIA is a simple, objective, and reproducible method for the detection of antibodies to CMV in sera, plasma, or whole blood samples, and the total procedure is adaptable for batching and automation and takes only 3 hr of elapsed time.

摘要

五家临床机构的研究人员对一种用于检测巨细胞病毒（CMV）抗体存在与否的间接酶免疫测定法（CMV总抗体酶免疫测定法，雅培实验室）进行了评估。批间和批内变异系数数据表明，酶免疫测定法在所有机构均能以高度可重复的方式进行。研究人员通过CMV总抗体酶免疫测定法和间接血凝试验（IHA）对总共2314份临床样本进行了CMV抗体检测。总体结果表明，与IHA相比，酶免疫测定法的灵敏度为97.9%（1096/1120），特异性为93.6%（1096/1171）。检测的临床样本包括血清、血浆和全血。这些结果表明CMV总抗体酶免疫测定法与IHA之间具有良好的一致性。这种酶免疫测定法是一种简单、客观且可重复的方法，用于检测血清、血浆或全血样本中的CMV抗体，整个过程适用于批量检测和自动化，耗时仅3小时。