Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Kitakyushu Science and Research Park, Kitakyushu, Fukuoka, Japan.
Biochem Biophys Res Commun. 2018 Sep 5;503(2):910-914. doi: 10.1016/j.bbrc.2018.06.095. Epub 2018 Jun 23.
Ultraviolet (UV) radiation causes damage in all living organisms, including DNA damage that leads to cell death. Herein, we provide a new technique for UV radiation protection through intracellular short peptide expression. The late embryogenesis abundant (LEA) peptide, which functions as a shield that protects macromolecules from various abiotic stress, was obtained from the Polypedilum vanderplanki group 3 LEA protein. Recombinant Escherichia coli BL21 (DE3) expressing functional LEA short peptide in vivo were exposed to UVA and UVC radiation for 4, 6, and 8 h. E. coli transformants expressing the LEA peptide showed higher cell viability under both UVA and UVC treatment at all time points as compared with that of the control. Furthermore, the cells expressing LEA peptide showed a higher number of colony-forming units per dilution under UVA and UVC treatment. These results suggested that expression of the short peptide could be useful for the development of genetically modified organisms and in applications that require resilience of organisms to UV radiation.
紫外线（UV）辐射会对所有生物造成伤害，包括导致细胞死亡的 DNA 损伤。在此，我们通过细胞内短肽表达提供了一种新的 UV 辐射防护技术。晚期胚胎丰富（LEA）肽作为一种保护大分子免受各种非生物胁迫的盾牌，取自 Polypedilum vanderplanki 第 3 组 LEA 蛋白。在体内表达功能性 LEA 短肽的重组大肠杆菌 BL21（DE3）在 UVA 和 UVC 辐射下暴露 4、6 和 8 小时。与对照相比，在所有时间点，表达 LEA 肽的大肠杆菌转化体在 UVA 和 UVC 处理下显示出更高的细胞活力。此外，在 UVA 和 UVC 处理下，表达 LEA 肽的细胞每稀释度形成的菌落形成单位数量更多。这些结果表明，短肽的表达可能有助于开发转基因生物，并在需要生物体对 UV 辐射具有弹性的应用中发挥作用。
