Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu, Kitakyushu, 808-0196, Japan.
Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, 11241, Egypt.
Appl Biochem Biotechnol. 2020 May;191(1):164-176. doi: 10.1007/s12010-020-03262-5. Epub 2020 Feb 25.
Unsuitable pH is a major limiting factor for all organisms, and a low pH can lead to organism death. Late embryogenesis abundant (LEA) peptides confer tolerance to abiotic stresses including salinity, drought, high and low temperature, and ultraviolet radiation same as the LEA proteins from which they originate. In this study, LEA peptides derived from group 3 LEA proteins of Polypedilum vanderplanki were used to enhance low pH tolerance. Recombinant Escherichia coli BL21 (DE3) cells expressing the five designed LEA peptides were grown at pH 4, 3, and 2. The transformants showed higher growth capacity at low pH as compared to control cells. These results indicate that LEA peptide could prevent E. coli cell death under low pH conditions.
不适宜的 pH 值是所有生物体的主要限制因素，而低 pH 值可能导致生物体死亡。晚期胚胎丰富蛋白 (LEA) 肽能够耐受包括盐度、干旱、高低温以及紫外线辐射在内的非生物胁迫，这与它们起源的 LEA 蛋白相同。在这项研究中，使用源自 Polypedilum vanderplanki 第 3 组 LEA 蛋白的 LEA 肽来提高对低 pH 值的耐受性。在 pH 值为 4、3 和 2 下培养表达五个设计的 LEA 肽的重组大肠杆菌 BL21 (DE3) 细胞。与对照细胞相比，转化体在低 pH 值下显示出更高的生长能力。这些结果表明 LEA 肽可以防止大肠杆菌细胞在低 pH 值条件下死亡。
