Department of Pharmacy, Shanghai East Hospital, Tongji University, Shanghai, 200120, China.
Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, 200092, China.
Biochem Biophys Res Commun. 2018 Sep 5;503(2):729-736. doi: 10.1016/j.bbrc.2018.06.068. Epub 2018 Jun 21.
Mitochondria, the powerhouse of eukaryotic cells, are highly dynamic organelle. Mitochondrial fission, fusion, kissing and contraction have been reported over and over again in non-static cells, such as fibroblast, with tubular mitochondrial networks. Even though the fluorescence propagation among mitochondria of mature cardiomyocytes had been captured using mitochondrial matrix targeted photoactivatable GFP (PAGFP) or MitoDendra proteins, there are no direct evidence that single real time mitochondrial dynamics events exist in mature cardiomyocytes with ball-like mitochondria. Here we first time revealed the visualizable single mitochondrial dynamics events in adult mature cardiomyocytes by the mitochondrial flash (mitoflash). We found fission, fusion, contraction and kissing were accompanied by a mitoflash event. Metabolism could increase mitochondrial contraction. Fusion and Kissing mediated inter-mitochondrial communication with higher frequency than fission. These results demonstrate that mitochondria of static mature cardiomyocytes are undergoing the rare, but real dynamics change.
线粒体是真核细胞的“动力工厂”，是高度动态的细胞器。在非静止细胞（如成纤维细胞）中，人们反复报道了线粒体的分裂、融合、融合和收缩，这些细胞具有管状的线粒体网络。尽管已经使用线粒体基质靶向光激活 GFP（PAGFP）或 MitoDendra 蛋白在成熟心肌细胞的线粒体之间捕获了荧光传播，但没有直接证据表明在具有球状线粒体的成熟心肌细胞中存在单个实时线粒体动力学事件。在这里，我们首次通过线粒体闪光（mitoflash）揭示了成年成熟心肌细胞中可可视化的单个线粒体动力学事件。我们发现分裂、融合、收缩和融合伴随着线粒体闪光事件。代谢可以增加线粒体的收缩。融合和融合介导的线粒体间通讯的频率高于分裂。这些结果表明，静止成熟心肌细胞的线粒体正在经历罕见但真实的动力学变化。
