Department of Medical Oncology, Xuzhou Central Hospital, XuZhou, 221009, China.
Department of Medical Oncology, Xuzhou Central Hospital, XuZhou, 221009, China.
Biochem Biophys Res Commun. 2018 Sep 5;503(2):870-875. doi: 10.1016/j.bbrc.2018.06.089. Epub 2018 Jun 23.
Recently, circular RNA (circRNA) is identified as a novel class of noncoding RNA with important roles in human diseases, such as cancer. However, how circRNA participates in colorectal cancer (CRC) remains unclear. In this study, we aimed to illustrate the role of circ_0026344 in CRC progression. We showed that circ_0026344 expression was downregulated in CRC tissues compared to adjacent normal tissues. Moreover, the level of circ_0026344 was inversely correlated with CRC advance and lymphoid node metastasis. Additionally, circ_0026344 low expression predicted poor prognosis in CRC patients. We identified circ_0026344 as a miRNA sponge for microRNA-21 (miR-21) and microRNA-31 (miR-31) whose expression levels were elevated in CRC tissues. And the level of circ_0026344 was reversely correlated with both miR-21 and miR-31 levels in CRC tissues. Functionally, we found that ectopic expression of circ_0026344 decreased the growth and invasion of CRC cells while promoting apoptosis in vitro. The xenograft experiment indicated that circ_0026344 overexpress led to CRC growth inhibition in vivo. Rescue assays further demonstrated that circ_0026344 exerted biological functions by sponging miR-21 and miR-31 in CRC. In conclusion, this study revealed that circ_0026344/miR-21/miR-31 regulatory signaling was implicated in CRC progression.
最近，环状 RNA（circRNA）被鉴定为一类新的非编码 RNA，在人类疾病中具有重要作用，如癌症。然而，circRNA 如何参与结直肠癌（CRC）仍不清楚。在这项研究中，我们旨在阐明 circ_0026344 在 CRC 进展中的作用。我们显示，与相邻正常组织相比，circ_0026344 在 CRC 组织中的表达下调。此外，circ_0026344 的水平与 CRC 进展和淋巴结转移呈负相关。此外，circ_0026344 低表达预示 CRC 患者预后不良。我们发现 circ_0026344 是 microRNA-21（miR-21）和 microRNA-31（miR-31）的 miRNA 海绵，其在 CRC 组织中的表达水平升高。并且 circ_0026344 的水平与 CRC 组织中 miR-21 和 miR-31 的水平呈负相关。功能上，我们发现外源性表达 circ_0026344 可降低 CRC 细胞的生长和侵袭，同时促进体外细胞凋亡。异种移植实验表明，circ_0026344 过表达可抑制体内 CRC 的生长。挽救实验进一步表明，circ_0026344 通过在 CRC 中海绵吸附 miR-21 和 miR-31 发挥生物学功能。总之，这项研究表明 circ_0026344/miR-21/miR-31 调节信号参与了 CRC 的进展。
