Department of Obstetrics and Gynecology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China.
Department of Obstetrics and Gynecology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, 310006, China.
Microb Pathog. 2018 Oct;123:139-143. doi: 10.1016/j.micpath.2018.06.031. Epub 2018 Jun 19.
Endometritis is a puzzling disease that often associates with severe pelvic pain. In this study, we aimed to detect whether apigenin had protective effect against LPS-induced endometritis, if so, the underlying mechanism was further investigated. Apigenin was administrated 1 h before LPS treatment. The levels of inflammatory cytokines were measured by ELISA. The expression of NF-κB and Nrf2 were detected by Western blot analysis. The results showed that LPS treatment induced severe histological alteration of uterus and this change was attenuated by the treatment of apigenin. Apigenin significantly attenuated LPS-induced MPO activity, MDA content, and inflammatory cytokines TNF-α and IL-1β production. LPS-induced NF-κB activation was suppressed by apigenin. Furthermore, apigenin elevated the expression of Nrf2 and HO-1 in uterine tissues. In conclusion, the present study demonstrated that apigenin protected against LPS-induced endometritis through activation of Nrf2 signaling pathway and inhibition of NF-κB signaling pathway.
子宫内膜炎是一种令人困惑的疾病，常伴有严重的盆腔疼痛。在这项研究中，我们旨在检测芹菜素是否对 LPS 诱导的子宫内膜炎有保护作用，如果有，进一步探讨其潜在机制。在 LPS 处理前 1 小时给予芹菜素。通过 ELISA 法测定炎症细胞因子的水平。通过 Western blot 分析检测 NF-κB 和 Nrf2 的表达。结果表明，LPS 处理诱导子宫严重的组织学改变，而芹菜素处理可减轻这种改变。芹菜素显著减轻 LPS 诱导的 MPO 活性、MDA 含量以及 TNF-α和 IL-1β的产生。芹菜素抑制 LPS 诱导的 NF-κB 激活。此外，芹菜素增加了子宫组织中 Nrf2 和 HO-1 的表达。总之，本研究表明，芹菜素通过激活 Nrf2 信号通路和抑制 NF-κB 信号通路来保护 LPS 诱导的子宫内膜炎。
