School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur, India.
Vishnu Cancer Center, Thanjavur, Tamil Nadu, India.
Toxicol In Vitro. 2018 Oct;52:170-177. doi: 10.1016/j.tiv.2018.06.015. Epub 2018 Jun 19.
Radiotherapy is the most widely used treatment method for treating cancer with or without surgery and chemotherapy. In lung cancer, it is one of the important treatment steps in excising the tumor from the lung tissue; unfortunately, radiation can induce epithelial- mesenchymal transition (EMT), a typical physiological process in which cuboidal shaped epithelial cell loses its phenotype and acquires mesenchymal-like phenotype thus, increases the metastasis progression in the body. To prevent EMT mediated metastasis, we aimed to 1) synthesize silver nanoparticles by using Gallic acid, a potential antioxidant which acts as stabilizing and reducing agent in the form of silver nanoparticle (GA-AgNPs) 2) to analyze its effect on EMT markers during radiation-induced EMT in A549 cells.
A549 cells were irradiated with 8Gy (X-ray) and treated with GA-AgNPs at a fixed concentration under in vitro condition. GA-AgNPs were prepared and characterized for absorption, potential stability, size and morphology by UV-Visible spectrophotometer, Zeta potential and Transmission electron microscopy respectively. After irradiation, the morphology changes were observed using an inverted microscope, the gene and protein expression of EMT markers were analyzed by RT-PCR and western blotting.
RESULTS/CONCLUSION: GA-AgNPs are in nano size with fair stability. The synthesized nanoparticles suppressed the EMT markers including Vimentin, N-cadherin, Snail-1 and increased E-cadherin expression which might inhibit cancer cells to acquire radio resistant metastasis potential.
放疗是治疗癌症的最广泛使用的方法，无论是否有手术和化疗。在肺癌中，它是从肺部组织切除肿瘤的重要治疗步骤之一；不幸的是，辐射会诱导上皮-间充质转化（EMT），这是一种典型的生理过程，其中立方形状的上皮细胞失去其表型并获得间充质样表型，从而增加了体内的转移进展。为了防止 EMT 介导的转移，我们旨在 1）通过使用没食子酸合成银纳米粒子，没食子酸是一种潜在的抗氧化剂，以银纳米粒子（GA-AgNPs）的形式作为稳定剂和还原剂；2）分析其在 A549 细胞辐射诱导的 EMT 过程中对 EMT 标志物的影响。
在体外条件下，用 8Gy（X 射线）照射 A549 细胞，并在固定浓度下用 GA-AgNPs 处理。通过紫外可见分光光度计、Zeta 电位和透射电子显微镜分别对 GA-AgNPs 的吸收、潜在稳定性、大小和形态进行了分析。照射后，用倒置显微镜观察形态变化，用 RT-PCR 和 Western blot 分析 EMT 标志物的基因和蛋白表达。
结果/结论：GA-AgNPs 为纳米尺寸，稳定性良好。合成的纳米粒子抑制了 EMT 标志物，包括波形蛋白、N-钙黏蛋白、Snail-1，并增加了 E-钙黏蛋白的表达，这可能抑制癌细胞获得放射抵抗转移的潜力。
