Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland.
Department of Chemistry, Wroclaw University of Science and Technology, Norwida 4, 50-373 Wroclaw, Poland.
Bioelectrochemistry. 2018 Oct;123:255-259. doi: 10.1016/j.bioelechem.2018.06.005. Epub 2018 Jun 6.
There are still not specified mechanisms how beta-glucan molecules are transported into cells. Supposing, beta-glucan toxicity against tumor cells may be related to the overexpression of the transporter responsible for the transport of glucose molecules in the cells. In this case, glucans - polymers composed of glucose units are much more up-taken by tumor than normal cells. Increased GLUT1 (Glucose Transporter Type 1) expression has been demonstrated earlier in malignant melanomas. GLUT1 expression promotes glucose uptake and cell growth in that cells. Also, in human melanoma tissues a significant correlation between GLUT1 expression and mitotic activity was found. The aim of the study was to verify if oat β-glucan (OβG) is delivered into cells by GLUT-1 membrane protein. To check it out we blocked GLUT1 transporters by an inhibitor WZB117 and then we investigated cells viability with and without reversible electroporation (EP). The obtained results bring us to elucidate the mechanism of transport of the OβG into the cells is GLUT-1 dependent and moreover can be supported by EP method.
目前还没有明确的机制可以解释β-葡聚糖分子是如何进入细胞的。假设β-葡聚糖对肿瘤细胞的毒性可能与负责细胞内葡萄糖分子转运的转运体的过度表达有关。在这种情况下，葡聚糖——由葡萄糖单元组成的聚合物，比正常细胞更容易被肿瘤细胞吸收。先前已经证明恶性黑色素瘤中 GLUT1（葡萄糖转运蛋白 1 型）的表达增加。GLUT1 表达促进葡萄糖摄取和细胞生长。此外，在人类黑色素瘤组织中发现 GLUT1 表达与有丝分裂活性之间存在显著相关性。本研究的目的是验证燕麦β-葡聚糖（OβG）是否通过 GLUT-1 膜蛋白进入细胞。为了验证这一点，我们用抑制剂 WZB117 阻断 GLUT1 转运体，然后在有无可逆电穿孔（EP）的情况下研究细胞活力。研究结果表明，OβG 进入细胞的运输机制依赖于 GLUT-1，并且可以通过 EP 方法得到支持。
