Department of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, 9 M. Skłodowska-Curie St., 85-094 Bydgoszcz, Poland.
Department of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, 9 M. Skłodowska-Curie St., 85-094 Bydgoszcz, Poland.
Int J Food Microbiol. 2018 Oct 3;282:71-83. doi: 10.1016/j.ijfoodmicro.2018.06.011. Epub 2018 Jun 13.
The aim of this research was to investigate the occurrence of Listeria monocytogenes in fish and fish processing plant and to determine their transmission, virulence and antibiotic resistance. L. monocytogenes was isolated according to the ISO 11290-1. The identification of L. monocytogenes was confirmed by multiplex PCR method. Genetic similarity of L. monocytogenes strains was determined with the Pulsed-Filed Gene Electrophoresis (PFGE) method. The multiplex PCR was used for identification of L. monocytogenes serogroups and detection of selected virulence genes (actA, fbpA, hlyA, iap, inlA, inlB, mpl, plcA, plcB, prfA). The L. monocytogens isolates susceptibility to penicillin, ampicillin, meropenem, erythromycin, trimethoprim/sulfamethoxazole was evaluated with disc diffusion method according to EUCAST v. 7.1. The presence of 237 L. monocytogenes isolates (before genetic similarity assessment) in 614 examined samples was confirmed. After strain differentiation by PFGE techniques the presence of 161 genetically different strains were confirmed. The genetic similarity of the examined isolates suggested that the source of the L. monocytogenes strains were fishes originating from farms. All tested strains possessed all detected virulence genes. Among examined strains, the most (26, 38.6%) belonged to the group 1/2a-3a. The most of tested strains were resistant to erythromycin (47.1%) and trimethoprim/sulfamethoxazole (47.1%).
本研究旨在调查鱼类和鱼类加工厂中李斯特菌的发生情况，并确定其传播途径、毒力和抗生素耐药性。根据 ISO 11290-1 分离李斯特菌。通过多重 PCR 方法确认李斯特菌的鉴定。采用脉冲场凝胶电泳（PFGE）方法确定李斯特菌菌株的遗传相似性。多重 PCR 用于鉴定李斯特菌血清群和检测选定的毒力基因（actA、fbpA、hlyA、iap、inlA、inlB、mpl、plcA、plcB、prfA）。采用 EUCAST v. 7.1 中的纸片扩散法评估青霉素、氨苄西林、美罗培南、红霉素、甲氧苄啶/磺胺甲恶唑对李斯特菌分离株的敏感性。在 614 个检查样本中确认了 237 株李斯特菌（在进行遗传相似性评估之前）的存在。通过 PFGE 技术对菌株进行分化后，确认存在 161 种遗传上不同的菌株。检查分离株的遗传相似性表明，李斯特菌菌株的来源是来自农场的鱼类。所有测试菌株均携带所有检测到的毒力基因。在检查的菌株中，大多数（26，38.6%）属于 1/2a-3a 组。大多数测试菌株对红霉素（47.1%）和甲氧苄啶/磺胺甲恶唑（47.1%）耐药。
