Inglis Peter W, Mata Lorena R, da Silva Marcos José, Vieira Roberto F, Alves Rosa de B N, Silva Dijalma B, Azevedo Vânia C R
Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Brasília-DF, Brazil.
Universidade Federal de Goiás, Goiânia-GO, Brazil.
Planta Med. 2018 Nov;84(17):1300-1310. doi: 10.1055/a-0644-2688. Epub 2018 Jun 21.
Plants of the genus principally , and are used in Brazilian folk medicine to treat kidney stones as well as other ailments, where the latter two species are listed in the Brazilian Pharmacopeia as (). However, only has been shown to be effective in a clinical setting. Nuclear ribosomal internal transcribed spacer (ITS1 - 5.8S rRNA-ITS2), internal transcribed spacer 2, and chloroplasts L, K, A-H, L, and L-F were screened for their potential as DNA barcodes for the identification of 48 taxa in Brazilian medicinal plant germplasm banks and in "living pharmacies". The markers were also tested for their ability to validate four commercial herbal teas labelled as . Using the criterion of high clade posterior probability in Bayesian phylogenetic analysis, the internal transcribed spacer, internal transcribed spacer 2, and chloroplast K, A-H, L, and L-F markers all reliably differentiated the four species, with the internal transcribed spacer and K possessing the additional advantage that the genus is well represented for these markers in the Genbank database. However, in the case of L, posterior probability for some clades was low and while and formed monophyletic groups, and accessions could not be reliably distinguished with this marker. Packaged dried herb from three Brazilian commercial suppliers comprised , but one sample was also found to be mixed with alfalfa (). An herb marketed as from a fourth supplier was found to be composed of a mixture of and .
该属植物主要是[具体植物名称1]和[具体植物名称2]，在巴西民间医学中用于治疗肾结石及其他疾病，后两种植物在《巴西药典》中被列为[相关类别]。然而，只有[具体植物名称1]在临床环境中被证明是有效的。对核糖体DNA内转录间隔区（ITS1 - 5.8S rRNA - ITS2）、内转录间隔区2以及叶绿体L、K、A - H、L和L - F进行了筛选，以评估它们作为DNA条形码用于鉴定巴西药用植物种质库和“活体药房”中48个[植物属名]分类群的潜力。这些标记还被测试用于验证四种标为[相关名称]的商业凉茶的能力。根据贝叶斯系统发育分析中高分支后验概率的标准，内转录间隔区、内转录间隔区2以及叶绿体K、A - H、L和L - F标记均能可靠地区分这四种[植物属名]物种，内转录间隔区和K具有额外的优势，即该属在Genbank数据库中这些标记的代表性良好。然而，对于L标记，一些分支的后验概率较低，虽然[具体植物名称3]和[具体植物名称4]形成了单系群，但使用该标记无法可靠地区分[具体植物名称5]和[具体植物名称6]的种质。来自巴西三个商业供应商的包装干燥[植物名称1]草药包含[具体成分1]，但还发现一个样品与苜蓿（[苜蓿学名]）混合。从第四个供应商处销售的标为[相关名称]的一种草药被发现由[具体植物名称1]和[具体植物名称7]的混合物组成。
