Nitsch L, Tramontano D, Ambesi-Impiombato F S, Quarto N, Bonatti S
Eur J Cell Biol. 1985 Jul;38(1):57-66.
The thyroid epithelial cell line FRT in monolayer culture appeared to be strongly polarized by morphological criteria. Cells were connected by tight junctions, exposed microvilli toward the culture medium and formed domes at confluency. FRT cells were infected with vesicular stomatitis virus (VSV) and Sindbis virus and the budding polarity was examined 8 and 16 h after infection, respectively. VSV budding occurred preferentially from the basolateral domain of plasma membrane, while Sindbis virus budding was mostly apical. The distribution of VSV and Sindbis virus glycoproteins, as determined by the immuno-gold technique, correlated well with the budding polarity. Polarized budding was not observed in isolated cells in suspension.
单层培养的甲状腺上皮细胞系FRT在形态学标准上似乎具有很强的极性。细胞通过紧密连接相连，向培养基方向伸出微绒毛，并在汇合时形成穹顶。用水泡性口炎病毒（VSV）和辛德毕斯病毒感染FRT细胞，并分别在感染后8小时和16小时检查出芽极性。VSV出芽优先发生在质膜的基底外侧结构域，而辛德毕斯病毒出芽大多发生在顶端。通过免疫金技术测定的VSV和辛德毕斯病毒糖蛋白的分布与出芽极性密切相关。在悬浮的分离细胞中未观察到极化出芽。
