Zurzolo C, Polistina C, Saini M, Gentile R, Aloj L, Migliaccio G, Bonatti S, Nitsch L
Centro di Endocrinologia ed Oncologia Sperimentale del Consiglio Nazionale delle Ricerche, Dipartimento di Biologia e Patologia Cellulare e Naples, Italy.
J Cell Biol. 1992 May;117(3):551-64. doi: 10.1083/jcb.117.3.551.
We compared the surface envelope glycoprotein distribution and the budding polarity of four RNA viruses in Fischer rat thyroid (FRT) cells and in CaCo-2 cells derived from a human colon carcinoma. Whereas both FRT and CaCo-2 cells sort similarly influenza hemagglutinin and vesicular stomatitis virus (VSV) G protein, respectively, to apical and basolateral membrane domains, they differ in their handling of two togaviruses, Sindbis and Semliki Forest virus (SFV). By conventional EM Sindbis virus and SFV were shown to bud apically in FRT cells and basolaterally in CaCo-2 cells. Consistent with this finding, the distribution of the p62/E2 envelope glycoprotein of SFV, assayed by immunoelectronmicroscopy and by domain-selective surface biotinylation was predominantly apical on FRT cells and basolateral on CaCo-2 cells. We conclude that a given virus and its envelope glycoprotein can be delivered to opposite membrane domains in epithelial cells derived from different tissues. The tissue specificity in the polarity of virus budding and viral envelope glycoprotein distribution indicate that the sorting machinery varies considerably between different epithelial cell types.
我们比较了四种RNA病毒在Fischer大鼠甲状腺（FRT）细胞和源自人结肠癌的CaCo-2细胞中的表面包膜糖蛋白分布及出芽极性。虽然FRT细胞和CaCo-2细胞分别将流感血凝素和水疱性口炎病毒（VSV）G蛋白相似地分选到顶端和基底外侧膜结构域，但它们在处理两种披膜病毒，即辛德毕斯病毒和塞姆利基森林病毒（SFV）时存在差异。通过传统电子显微镜观察发现，辛德毕斯病毒和SFV在FRT细胞中顶端出芽，而在CaCo-2细胞中基底外侧出芽。与此发现一致，通过免疫电子显微镜和结构域选择性表面生物素化检测的SFV的p62/E2包膜糖蛋白分布在FRT细胞上主要位于顶端，在CaCo-2细胞上位于基底外侧。我们得出结论，给定的病毒及其包膜糖蛋白可以被递送到源自不同组织的上皮细胞的相反膜结构域。病毒出芽极性和病毒包膜糖蛋白分布的组织特异性表明，不同上皮细胞类型之间的分选机制差异很大。
