• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

普遍保守的 GTPase HflX 是一种 RNA 解旋酶，可修复受热损伤的核糖体。

The universally conserved GTPase HflX is an RNA helicase that restores heat-damaged ribosomes.

机构信息

Structural Biology and Bioinformatics Division, Council of Scientific and Industrial Research - Indian Institute of Chemical Biology, Kolkata, India.

Structural Biology and Bioinformatics Division, Council of Scientific and Industrial Research - Indian Institute of Chemical Biology, Kolkata, India

出版信息

J Cell Biol. 2018 Jul 2;217(7):2519-2529. doi: 10.1083/jcb.201711131. Epub 2018 Jun 21.

DOI:10.1083/jcb.201711131
PMID:29930203
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6028529/
Abstract

The ribosome-associated GTPase HflX acts as an antiassociation factor upon binding to the 50S ribosomal subunit during heat stress in Although HflX is recognized as a guanosine triphosphatase, several studies have shown that the N-terminal domain 1 of HflX is capable of hydrolyzing adenosine triphosphate (ATP), but the functional role of its adenosine triphosphatase (ATPase) activity remains unknown. We demonstrate that HflX possesses ATP-dependent RNA helicase activity and is capable of unwinding large subunit ribosomal RNA. A cryo-electron microscopy structure of the 50S-HflX complex in the presence of nonhydrolyzable analogues of ATP and guanosine triphosphate hints at a mode of action for the RNA helicase and suggests the linker helical domain may have a determinant role in RNA unwinding. Heat stress results in inactivation of the ribosome, and we show that HflX can restore heat-damaged ribosomes and improve cell survival.

摘要

核糖体相关 GTP 酶 HflX 在热应激期间与 50S 核糖体亚基结合，充当抗聚集因子。尽管 HflX 被认为是一种鸟苷三磷酸酶，但有几项研究表明 HflX 的 N 端结构域 1 能够水解三磷酸腺苷 (ATP)，但其三磷酸腺苷酶 (ATPase) 活性的功能作用尚不清楚。我们证明 HflX 具有依赖于 ATP 的 RNA 解旋酶活性，并能够解旋大亚基核糖体 RNA。在存在非水解型 ATP 和鸟苷三磷酸类似物的情况下，50S-HflX 复合物的冷冻电子显微镜结构提示了 RNA 解旋酶的作用模式，并表明连接螺旋结构域可能在 RNA 解旋中起决定作用。热应激导致核糖体失活，我们表明 HflX 可以恢复受热损伤的核糖体并提高细胞存活率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7cb/6028529/ab28d1ab1945/JCB_201711131_Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7cb/6028529/881e76cf87d6/JCB_201711131_GA.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7cb/6028529/e344f546b04a/JCB_201711131_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7cb/6028529/33208f774002/JCB_201711131_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7cb/6028529/846f542ea684/JCB_201711131_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7cb/6028529/0068da35e5f4/JCB_201711131_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7cb/6028529/57ad3e841a7b/JCB_201711131_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7cb/6028529/ab28d1ab1945/JCB_201711131_Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7cb/6028529/881e76cf87d6/JCB_201711131_GA.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7cb/6028529/e344f546b04a/JCB_201711131_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7cb/6028529/33208f774002/JCB_201711131_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7cb/6028529/846f542ea684/JCB_201711131_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7cb/6028529/0068da35e5f4/JCB_201711131_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7cb/6028529/57ad3e841a7b/JCB_201711131_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7cb/6028529/ab28d1ab1945/JCB_201711131_Fig6.jpg

相似文献

1
The universally conserved GTPase HflX is an RNA helicase that restores heat-damaged ribosomes.普遍保守的 GTPase HflX 是一种 RNA 解旋酶，可修复受热损伤的核糖体。
J Cell Biol. 2018 Jul 2;217(7):2519-2529. doi: 10.1083/jcb.201711131. Epub 2018 Jun 21.
2
E. coli HflX interacts with 50S ribosomal subunits in presence of nucleotides.在核苷酸存在的情况下，大肠杆菌HflX与50S核糖体亚基相互作用。
Biochem Biophys Res Commun. 2009 Feb 6;379(2):201-5. doi: 10.1016/j.bbrc.2008.12.072. Epub 2008 Dec 25.
3
The ribosome modulates the structural dynamics of the conserved GTPase HflX and triggers tight nucleotide binding.核糖体调节保守 GTP 酶 HflX 的结构动力学，并触发紧密的核苷酸结合。
Biochimie. 2012 Aug;94(8):1647-59. doi: 10.1016/j.biochi.2012.04.016. Epub 2012 Apr 25.
4
The conserved GTPase HflX is a ribosome splitting factor that binds to the E-site of the bacterial ribosome.保守的GTP酶HflX是一种核糖体分裂因子，它与细菌核糖体的E位点结合。
Nucleic Acids Res. 2016 Feb 29;44(4):1952-61. doi: 10.1093/nar/gkv1524. Epub 2016 Jan 4.
5
Properties of HflX, an enigmatic protein from Escherichia coli.来自大肠杆菌的神秘蛋白质HflX的特性
J Bacteriol. 2009 Apr;191(7):2307-14. doi: 10.1128/JB.01353-08. Epub 2009 Jan 30.
6
Structural modules of the stress-induced protein HflX: an outlook on its evolution and biological role.应激诱导蛋白HflX的结构模块：对其进化和生物学作用的展望
Curr Genet. 2019 Apr;65(2):363-370. doi: 10.1007/s00294-018-0905-x. Epub 2018 Nov 17.
7
Toward understanding the function of the universally conserved GTPase HflX from Escherichia coli: a kinetic approach.深入了解大肠杆菌中普遍保守的GTP酶HflX的功能：一种动力学方法。
Biochemistry. 2009 Nov 17;48(45):10793-802. doi: 10.1021/bi901074h.
8
Chlamydophila pneumoniae HflX belongs to an uncharacterized family of conserved GTPases and associates with the Escherichia coli 50S large ribosomal subunit.肺炎衣原体HflX属于一个尚未明确特征的保守GTP酶家族，并与大肠杆菌50S大核糖体亚基相关联。
Microbiology (Reading). 2008 Nov;154(Pt 11):3537-3546. doi: 10.1099/mic.0.2008/022137-0.
9
The tandem GTPase, Der, is essential for the biogenesis of 50S ribosomal subunits in Escherichia coli.串联GTP酶Der对大肠杆菌50S核糖体亚基的生物合成至关重要。
Mol Microbiol. 2006 Sep;61(6):1660-72. doi: 10.1111/j.1365-2958.2006.05348.x. Epub 2006 Aug 23.
10
HflX is a ribosome-splitting factor rescuing stalled ribosomes under stress conditions.HflX 是一种核糖体分裂因子，可在应激条件下拯救停滞的核糖体。
Nat Struct Mol Biol. 2015 Nov;22(11):906-13. doi: 10.1038/nsmb.3103. Epub 2015 Oct 12.

引用本文的文献

1
Ribosome Purification, Co-sedimentation, and Subunit Association Assay.核糖体纯化、共沉降及亚基缔合分析
Bio Protoc. 2025 May 20;15(10):e5318. doi: 10.21769/BioProtoc.5318.
2
Mechanistic insights into the alternative ribosome recycling by HflXr.关于HflXr介导的替代性核糖体循环的机制性见解。
Nucleic Acids Res. 2024 Apr 24;52(7):4053-4066. doi: 10.1093/nar/gkae128.
3
Time resolution in cryo-EM using a PDMS-based microfluidic chip assembly and its application to the study of HflX-mediated ribosome recycling.使用基于 PDMS 的微流控芯片组件实现 cryo-EM 的时间分辨率及其在 HflX 介导的核糖体回收研究中的应用。

本文引用的文献

1
Sen1 has unique structural features grafted on the architecture of the Upf1-like helicase family.Sen1具有独特的结构特征，这些特征嫁接到了类Upf1解旋酶家族的结构体系上。
EMBO J. 2017 Jun 1;36(11):1590-1604. doi: 10.15252/embj.201696174. Epub 2017 Apr 13.
2
Modular Assembly of the Bacterial Large Ribosomal Subunit.细菌大核糖体亚基的模块化组装
Cell. 2016 Dec 1;167(6):1610-1622.e15. doi: 10.1016/j.cell.2016.11.020.
3
The conserved GTPase HflX is a ribosome splitting factor that binds to the E-site of the bacterial ribosome.保守的GTP酶HflX是一种核糖体分裂因子，它与细菌核糖体的E位点结合。
Cell. 2024 Feb 1;187(3):782-796.e23. doi: 10.1016/j.cell.2023.12.027. Epub 2024 Jan 19.
4
Insights into the function of the chloroplastic ribosome-associated GTPase high frequency of lysogenization X in .对叶绿体核糖体相关GTP酶高频溶原化X功能的见解。
Plant Direct. 2024 Jan 11;8(1):e559. doi: 10.1002/pld3.559. eCollection 2024 Jan.
5
Time resolution in cryo-EM using a novel PDMS-based microfluidic chip assembly and its application to the study of HflX-mediated ribosome recycling.使用新型基于聚二甲基硅氧烷（PDMS）的微流控芯片组件的冷冻电镜中的时间分辨率及其在HflX介导的核糖体循环研究中的应用
bioRxiv. 2023 Jul 29:2023.01.25.525430. doi: 10.1101/2023.01.25.525430.
6
Structure of Escherichia coli heat shock protein Hsp15 in complex with the ribosomal 50S subunit bearing peptidyl-tRNA.大肠杆菌热休克蛋白 Hsp15 与携带肽酰-tRNA 的核糖体 50S 亚基复合物的结构。
Nucleic Acids Res. 2022 Nov 28;50(21):12515-12526. doi: 10.1093/nar/gkac1035.
7
Structural basis for HflXr-mediated antibiotic resistance in Listeria monocytogenes.李斯特菌中介导抗生素耐药性的 HflXr 的结构基础。
Nucleic Acids Res. 2022 Oct 28;50(19):11285-11300. doi: 10.1093/nar/gkac934.
8
Delineating specific regions of N- terminal domain of T3SS ATPase YsaN of governing its different oligomerization states.描绘III型分泌系统ATP酶YsaN的N端结构域中控制其不同寡聚化状态的特定区域。
Front Mol Biosci. 2022 Sep 8;9:967974. doi: 10.3389/fmolb.2022.967974. eCollection 2022.
9
Maintaining mitochondrial ribosome function: The role of ribosome rescue and recycling factors.维持线粒体核糖体功能：核糖体救援和回收因子的作用。
RNA Biol. 2022;19(1):117-131. doi: 10.1080/15476286.2021.2015561. Epub 2021 Dec 31.
10
The Stringent Response Inhibits 70S Ribosome Formation in by Impeding GTPase-Ribosome Interactions.严格响应通过阻碍 GTPase-核糖体相互作用来抑制 的 70S 核糖体形成。
mBio. 2021 Dec 21;12(6):e0267921. doi: 10.1128/mBio.02679-21. Epub 2021 Nov 9.
Nucleic Acids Res. 2016 Feb 29;44(4):1952-61. doi: 10.1093/nar/gkv1524. Epub 2016 Jan 4.
4
HflX is a ribosome-splitting factor rescuing stalled ribosomes under stress conditions.HflX 是一种核糖体分裂因子，可在应激条件下拯救停滞的核糖体。
Nat Struct Mol Biol. 2015 Nov;22(11):906-13. doi: 10.1038/nsmb.3103. Epub 2015 Oct 12.
5
Crystal structure of the Bloom's syndrome helicase indicates a role for the HRDC domain in conformational changes.布卢姆氏综合征解旋酶的晶体结构表明HRDC结构域在构象变化中发挥作用。
Nucleic Acids Res. 2015 May 26;43(10):5221-35. doi: 10.1093/nar/gkv373. Epub 2015 Apr 21.
6
RNA helicase proteins as chaperones and remodelers.作为伴侣蛋白和重塑蛋白的RNA解旋酶
Annu Rev Biochem. 2014;83:697-725. doi: 10.1146/annurev-biochem-060713-035546. Epub 2014 Mar 12.
7
Functional domains of the 50S subunit mature late in the assembly process.50S 亚基的功能域在组装过程的后期成熟。
Nucleic Acids Res. 2014 Mar;42(5):3419-35. doi: 10.1093/nar/gkt1295. Epub 2013 Dec 13.
8
Identification and characterization of a hitherto unknown nucleotide-binding domain and an intricate interdomain regulation in HflX-a ribosome binding GTPase.鉴定和描述 HflX-a 核糖体结合 GTP 酶中一个迄今未知的核苷酸结合域和复杂的域间调节
Nucleic Acids Res. 2013 Nov;41(20):9557-69. doi: 10.1093/nar/gkt705. Epub 2013 Aug 16.
9
RNA helicases: diverse roles in prokaryotic response to abiotic stress.RNA 解旋酶：在原核生物应对非生物胁迫中的多种作用。
RNA Biol. 2013 Jan;10(1):96-110. doi: 10.4161/rna.22638. Epub 2012 Oct 23.
10
The cation-dependent G-proteins: in a class of their own.阳离子依赖型 G 蛋白：自成一派。
FEBS Lett. 2012 Jul 30;586(16):2218-24. doi: 10.1016/j.febslet.2012.06.030. Epub 2012 Jun 28.