Arruda José-Alcides, Silva Leni-Verônica, Silva Leorik, Monteiro João-Luiz, Álvares Pamella, Silveira Marcia, Sobral Ana-Paula
DDS, MSc Student, School of Dentistry, Department of Oral Surgery and Pathology, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
DDS, MSc, PhD Student, Postgraduate Program in Oral Pathology, Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil.
J Clin Exp Dent. 2018 Jun 1;10(6):e542-e547. doi: 10.4317/jced.54528. eCollection 2018 Jun.
To identify the prevalence and clinicopathological profile of calcifying odontogenic cysts (COC) stored at an oral pathology service, and to analyze the immunoexpression of cyclooxygenase 2 (COX-2) and cyclin D1 (CD1) in these cysts.
After a retrospective analysis (1990-2016) carried out to identify cases of COC, a sample of 12 cases was selected for immunohistochemical analysis of COX-2 and CD1 by the immunoperoxidase technique. Protein expression was evaluated semiquantitatively by attributing a score of 0 to 3 (0 = no staining; 1 = 1-25%; 2 = 26-50%, and 3 = >51% immunopositive cells).
Twenty cases of COC were diagnosed over the study period. These cysts were more common in the posterior mandible and in men (male-to-female ratio of 1.2:1), with a mean age of 29.9 years. Among the 12 cases analyzed, immunoexpression of COX-2 was observed only in the inflammatory infiltrate in 50% of the cysts (n = 6). Protein CD1 was detected (score 1) in 66.6% of cases (n = 8), and COX-2 was negative in 50% (n = 6).
The prevalence of COC among all odontogenic cysts was 3.5%, representing an uncommon lesion. Immunohistochemical analysis suggested that COX-2 does not participate in lesion progression. The cell proliferation index of COC was low, as demonstrated by the expression of CD1, suggesting a proliferative profile compatible with more indolent lesions. Odontogenic cysts, odontogenic tumors, epidemiology, immunohistochemistry, cell proliferation.
确定存储于口腔病理科的牙源性钙化囊肿（COC）的患病率及临床病理特征，并分析这些囊肿中环氧合酶2（COX-2）和细胞周期蛋白D1（CD1）的免疫表达情况。
在进行回顾性分析（1990 - 2016年）以确定COC病例后，选取12例样本，采用免疫过氧化物酶技术对COX-2和CD1进行免疫组织化学分析。通过赋予0至3分（0 =无染色；1 = 1 - 25%；2 = 26 - 50%，3 = >51%免疫阳性细胞）对蛋白表达进行半定量评估。
在研究期间共诊断出20例COC。这些囊肿在下颌后部及男性中更为常见（男女比例为1.2:1），平均年龄为29.9岁。在分析的12例病例中，50%（n = 6）的囊肿仅在炎症浸润中观察到COX-2免疫表达。66.6%（n = 8）的病例检测到蛋白CD1（评分为1），50%（n = 6）的病例COX-2呈阴性。
COC在所有牙源性囊肿中的患病率为3.5%，是一种罕见病变。免疫组织化学分析表明COX-2不参与病变进展。如CD1表达所示，COC的细胞增殖指数较低，提示其增殖特征与更惰性的病变相符。牙源性囊肿、牙源性肿瘤、流行病学、免疫组织化学、细胞增殖。
