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细菌在窝沟封闭剂上的黏附:接触酸性饮料的影响。

Bacterial adhesion on fissure sealants: Effects of exposure to acidic drink.

作者信息

Colombo Marco, Dagna Alberto, Molino Domenico, Poggio Claudio, Maiolatesi Diego, Pietrocola Giampiero

机构信息

Department of Clinical-Surgical, Diagnostic and Pediatric Sciences - Section of Dentistry, University of Pavia, Pavia, Italy.

Department of Molecular Medicine, Unit of Biochemistry, University of Pavia, Pavia, Italy.

出版信息

J Clin Exp Dent. 2018 Jun 1;10(6):e574-e578. doi: 10.4317/jced.54818. eCollection 2018 Jun.

Abstract

BACKGROUND

Adherence of bacteria to teeth surface is considered an important step in the development of caries and the use of fissure sealants is crucial for the prevention of caries in occlusal surfaces of molars and premolars. The aim of this study was to investigate and compare the adherence of to different fissure sealants, after acidic drink exposure.

MATERIAL AND METHODS

The tested materials were Fissurit, Fissurit FX, Grandio Seal, Fuji Triage, Constic. Bacterial suspension was deposited onto each material and the adhesion was evaluated trough the colony forming units (CFUs) determination with or without acidic drink exposure.

RESULTS

The tested materials showed different behaviors with significant differences. Bacterial adherence values of the untreated materials were very dissimilar: Fuji Triage and Constic materials showed the better results (<0.05).

CONCLUSIONS

Surface alteration after acidic drink exposure, changed the bacterial adhesion (except for Grandio Seal): Fissurit, Fissurit FX and Fuji Triage decreased their susceptibility to be colonized by (<0.05); on the contrary, Constic increased up to ~4 times the bacterial adhesiveness respected to the untreated control (<0.05). Acidic drinks, bacterial adhesion, fissure sealants, Streptococcus mutans.

摘要

背景

细菌在牙齿表面的黏附被认为是龋齿发展的重要步骤,而使用窝沟封闭剂对于预防磨牙和前磨牙咬合面的龋齿至关重要。本研究的目的是调查和比较在酸性饮料暴露后,(某种细菌)对不同窝沟封闭剂的黏附情况。

材料与方法

测试材料有Fissurit、Fissurit FX、Grandio Seal、Fuji Triage、Constic。将细菌悬液沉积在每种材料上,并通过测定菌落形成单位(CFU)来评估有无酸性饮料暴露情况下的黏附情况。

结果

测试材料表现出不同的行为,存在显著差异。未处理材料的细菌黏附值差异很大:Fuji Triage和Constic材料表现出较好的结果(<0.05)。

结论

酸性饮料暴露后的表面改变改变了细菌黏附(Grandio Seal除外):Fissurit、Fissurit FX和Fuji Triage降低了被(某种细菌)定植的易感性(<0.05);相反,Constic相对于未处理的对照,细菌黏附性增加了约4倍(<0.05)。酸性饮料、细菌黏附、窝沟封闭剂、变形链球菌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a57/6005089/d8c09446076e/jced-10-e574-g001.jpg

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