Poggio Claudio, Vialba Lodovico, Marchioni Riccardo, Colombo Marco, Pietrocola Giampiero
Department of Clinical-Surgical, Diagnostic and Pediatric Sciences, Section of Dentistry, University of Pavia, Pavia, Italy.
Department of Molecular Medicine, Unit of Biochemistry, University of Pavia, Pavia, Italy.
Eur J Dent. 2018 Apr-Jun;12(2):204-209. doi: 10.4103/ejd.ejd_219_17.
The purpose of this research was to evaluate and compare bacterial adhesion on five esthetic restorative materials, three glass ionomer cements (GIC), and two GIC with coat. All the materials were considered after acidic drink exposure.
Thirty cylindrical sample of each of the 10 materials were prepared and then divided into three groups: group 1 (baseline), Group 2 (1 day in acidic soft drink), and Group 3 (7 days in acidic soft drink). Bacterial suspension of was cultured and deposited onto each material, and the adhesion was evaluated through the colony-forming units determination. One-way ANOVA and Bonferroni's tests were applied to estimate significant differences between the esthetic materials.
The highest amount of was recorded in Group 3 and the lowest in Group 1 (baseline). In general, the GIC showed bacterial adhesion values higher than the ones related to composites both in Group 2 than in Group 3. Acidic soft drinks lead a time-dependent degradation of restorative materials causing an increase of the surface rugosity. In fact, a general increase in cells adhesion to treated samples was observed.
The use of acidic soft drink resulted in a degradation of the surface layer of the restorative material with consequent increase of bacterial adhesion. The GIC can be considered a more friendly environment for bacterial adhesion. This is true in particular if acid substances have already deteriorated the surface.
本研究旨在评估和比较五种美学修复材料、三种玻璃离子水门汀（GIC）以及两种带涂层的GIC上的细菌黏附情况。所有材料均在暴露于酸性饮料后进行评估。
制备了10种材料中每种材料的30个圆柱形样本，然后将其分为三组：第1组（基线）、第2组（在酸性软饮料中浸泡1天）和第3组（在酸性软饮料中浸泡7天）。培养细菌悬液并将其沉积在每种材料上，通过菌落形成单位测定来评估黏附情况。应用单因素方差分析和邦费罗尼检验来估计美学材料之间的显著差异。
第3组中记录的[细菌名称未给出]数量最高，第1组（基线）中最低。总体而言，在第2组和第3组中，GIC显示出的细菌黏附值均高于复合材料相关的值。酸性软饮料导致修复材料随时间降解，从而使表面粗糙度增加。事实上，观察到处理过的样本上[细菌名称未给出]细胞的黏附普遍增加。
使用酸性软饮料会导致修复材料表层降解，进而增加细菌黏附。GIC可被认为是更有利于细菌黏附的环境。特别是当酸性物质已经使表面变质时，情况更是如此。
