Department of Genetics, Federal University of Rio de Janeiro, RJ, Brazil.
Mol Biol Evol. 2018 Sep 1;35(9):2334-2342. doi: 10.1093/molbev/msy133.
The main outcome of molecular dating, the timetree, provides crucial information for understanding the evolutionary history of lineages and is a requirement of several evolutionary analyses. Although essential, the estimation of divergence times from molecular data is frequently regarded as a complicated task. However, establishing biological timescales can be performed in a straightforward manner, even with large, genome-wide data sets. This protocol presents all the necessary steps to estimate a timetree in the program MEGA X. It also illustrates how the TimeTree resource can be a useful tool to obtain chronological information based on previous studies, therefore yielding calibration boundaries.
分子年代测定的主要结果，即时间树，为了解谱系的进化历史提供了关键信息，也是几种进化分析的要求。尽管估计分子数据的分歧时间是必不可少的，但它通常被认为是一项复杂的任务。然而，即使使用大型的全基因组数据集，也可以以简单的方式建立生物时间表。本方案介绍了在 MEGA X 程序中估计时间树所需的所有步骤。它还说明了如何使用 TimeTree 资源根据先前的研究获取时间信息，从而产生校准边界。
