Bioconjug Chem. 2018 Jul 18;29(7):2325-2331. doi: 10.1021/acs.bioconjchem.8b00300. Epub 2018 Jul 6.
Cerium oxide (IV) nanoparticles offer a high redox ability, while maintaining nontoxicity and high stability. Thus, dispersed nanoceria is a promising candidate as antioxidant material for human cells. In this work, we report on a fast and simple one-pot process that yield a final nanoparticle size of 2-4 nm in polar solvents such as water and alcohols. High boiling point solvents, namely, benzyl alcohol and triethylene glycol, are used to obtain high crystalline nanoparticles by thermal and microwave activation. Transmission electron microscopy investigations prove the narrow size distribution of the CeO nanoparticles and show that the shape can be tuned from spherical to cubic using an appropriate precursor. The main objective of this work is to produce nanoparticles, which are well-defined, biocompatible, and stable in highly concentrated colloidal solutions (up to 90 mM) for a long period of time to study their behavior as antioxidant agents in human cells under oxidative stress.
氧化铈（IV）纳米粒子具有高氧化还原能力，同时保持无毒和高稳定性。因此，分散的纳米氧化铈是一种很有前途的作为人类细胞抗氧化剂材料。在这项工作中，我们报告了一种快速而简单的一锅法工艺，在极性溶剂如水中和醇中得到最终的纳米粒子尺寸为 2-4nm。高沸点溶剂，如苄醇和三甘醇，通过热和微波激活来获得高结晶纳米粒子。透射电子显微镜研究证明了 CeO 纳米粒子的窄尺寸分布，并表明可以使用适当的前体制备从球形到立方体形貌的纳米粒子。这项工作的主要目的是制备具有良好定义、生物相容性和在高浓度胶体溶液（高达 90mM）中长时间稳定的纳米粒子，以研究它们在氧化应激下作为人类细胞抗氧化剂的行为。
