Singh Varinder, Krishan Pawan, Shri Richa
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, Punjab, India.
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, Punjab, 147002, India.
J Basic Clin Physiol Pharmacol. 2018 Jul 26;29(4):403-410. doi: 10.1515/jbcpp-2017-0070.
Background Oxidative stress is strongly implicated in ischemia reperfusion (IR)-mediated functional and neuronal impairment. Therefore, strategies employing antioxidants to reverse the damage due to IR are being investigated. Allium schoenoprasum L. is a culinary medicine whose antioxidant properties are well documented but whose neuroprotective potential has not been examined. Hence, the present study was designed to evaluate the effect of A. schoenoprasum leaf extract (ASLE) on functional deficit against IR-induced cerebral injury in mice. Methods Acute toxicity studies of ASLE were performed following the Organisation for Economic Co-operation and Development Guideline 423. IR injury was induced by bilateral common carotid artery occlusion (BCCAO) for 15 min followed by 24-h reperfusion. Animals were treated for 7 days with ASLE (200 and 400 mg/kg, p.o. once daily) after IR injury. Functional outcomes (memory and sensorimotor functions) were measured using Morris water maze and neurological severity score, respectively. Cerebral infarct size and oxidative stress (thiobarbituric acid reactive species (TBARS), reduced glutathione (GSH), and superoxide dismutase (SOD) activity) were measured in order to elucidate the neuroprotective mechanism of ASLE. Results No toxic effects of ASLE were observed in mice. Oral treatment with ASLE for 7 days significantly attenuated IR-mediated memory and sensorimotor function deficit in the animals. The extract also reduced the cerebral infarct size and rise in brain TBARS levels, and restored the GSH levels and SOD activity. Conclusions The results of the present study suggest that ASLE is safe and effective in improving functional outcomes. It demonstrates neuroprotective effect by enhancing the antioxidant defence against IR injury.
背景 氧化应激与缺血再灌注（IR）介导的功能和神经元损伤密切相关。因此，正在研究采用抗氧化剂来逆转IR所致损伤的策略。细香葱是一种烹饪用草药，其抗氧化特性已有充分记载，但其神经保护潜力尚未得到研究。因此，本研究旨在评估细香葱叶提取物（ASLE）对小鼠IR诱导的脑损伤所致功能缺陷的影响。方法 按照经济合作与发展组织指南423进行ASLE的急性毒性研究。通过双侧颈总动脉闭塞（BCCAO）15分钟，随后再灌注24小时诱导IR损伤。IR损伤后，动物用ASLE（200和400mg/kg，口服，每日一次）治疗7天。分别使用莫里斯水迷宫和神经严重程度评分来测量功能结果（记忆和感觉运动功能）。测量脑梗死面积和氧化应激（硫代巴比妥酸反应性物质（TBARS）、还原型谷胱甘肽（GSH）和超氧化物歧化酶（SOD）活性），以阐明ASLE的神经保护机制。结果 在小鼠中未观察到ASLE的毒性作用。用ASLE口服治疗7天可显著减轻动物中IR介导的记忆和感觉运动功能缺陷。该提取物还减小了脑梗死面积，降低了脑TBARS水平的升高，并恢复了GSH水平和SOD活性。结论 本研究结果表明，ASLE在改善功能结果方面是安全有效的。它通过增强抗氧化防御以抵抗IR损伤而表现出神经保护作用。
