使用从蛋黄中提取的鸡抗病毒抗体进行定量微量补体结合试验。
Quantitative microcomplement fixation tests using chicken anti-viral antibody extracted from egg yolk.
作者信息
Van Regenmortel M H, Burckard J
出版信息
J Virol Methods. 1985 Jul;11(3):217-23. doi: 10.1016/0166-0934(85)90110-7.
PMID:2993337
Abstract
Yolk immunoglobulins obtained from hens immunized with human rotavirus and two plant virus antigens were used in quantitative microcomplement fixation tests. Difficulties inherent in the use of avian complement were overcome by utilizing a mixture of the C1 component of chicken complement and guinea-pig complement devoid of its own C1. The method is suitable for detecting small quantities of viral antigen and antibody and for detecting antigenic activity present on peptide fragments of viral proteins.
摘要
从用人轮状病毒和两种植物病毒抗原免疫的母鸡中获得的卵黄免疫球蛋白，用于定量微量补体结合试验。通过使用鸡补体的C1成分和不含自身C1的豚鼠补体的混合物，克服了使用禽补体所固有的困难。该方法适用于检测少量病毒抗原和抗体，以及检测病毒蛋白肽片段上存在的抗原活性。
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