Carrer Victor, Guzmán Beatriz, Martí Meritxell, Alonso Cristina, Coderch Luisa
Department of Chemical and Surfactants Technology, Institute of Advanced Chemistry of Catalonia (IQAC-CSIC), 08304 Barcelona, Spain.
Pharmaceutics. 2018 Jun 21;10(3):73. doi: 10.3390/pharmaceutics10030073.
The major in vitro permeation studies are currently performed in Franz-type diffusion cells because of their simplicity, cost effectiveness and because the experimental conditions can be easily controlled. Apart from the skin, Franz-type diffusion cells can be used with synthetic membranes. Nevertheless, they do not emulate the nature of the lipidic matrix, which is responsible for the topical barrier function.
This paper offers two new approaches combining different synthetic membranes (Strat-M and Nucleopore) with lanolin, which provides lipidic components similar to the lipidic matrix.
The molecular structure of lanolin was studied in membranes by attenuated total reflectance infrared spectroscopy (ATR-IR). The water permeability and absorption of lidocaine, diclofenac sodium and betamethasone dipropionate were also studied and compared against free-lanolin membranes and skin.
The results showed an increasing barrier function after lanolin application in both membranes, resulting in a decrease in water permeability. Observing the IR spectra, the lateral packaging of the lipid in the synthetic membranes seems to emulate the orthorhombic disposition from the stratum corneum. Moreover, the three substances applied to the lanolin-containing membranes have a similar absorption to that of the skin.
In conclusion, combining synthetic membranes with lanolin may be a useful approach to mimic topical actives’ absorption.
目前，主要的体外渗透研究是在弗兰兹型扩散池中进行的，因为其操作简单、成本效益高，且实验条件易于控制。除皮肤外，弗兰兹型扩散池还可用于合成膜。然而，它们无法模拟负责局部屏障功能的脂质基质的性质。
本文提出了两种新方法，将不同的合成膜（Strat-M和核孔膜）与羊毛脂相结合，羊毛脂可提供与脂质基质相似的脂质成分。
通过衰减全反射红外光谱（ATR-IR）研究了羊毛脂在膜中的分子结构。还研究了利多卡因、双氯芬酸钠和丙酸倍氯米松的透水性和吸收情况，并与游离羊毛脂膜和皮肤进行了比较。
结果表明，在两种膜中应用羊毛脂后，屏障功能增强，导致透水性降低。观察红外光谱可知，合成膜中脂质的横向排列似乎模拟了角质层的正交排列。此外，应用于含羊毛脂膜的三种物质的吸收情况与皮肤相似。
总之，将合成膜与羊毛脂相结合可能是模拟局部活性物质吸收的一种有用方法。
