Liu Rui-Ying, Zhang Si-Yuan, Ren Bin, He Yu-Man, Gu Jian, Tan Rui
College of Life Science and Engineering, Southwest Jaotong University, Chengdu 610031, China.
School of Pharmacy, Southwest University for Nationalities, Chengdu 610051, China.
Zhongguo Zhong Yao Za Zhi. 2018 May;43(10):2129-2133. doi: 10.19540/j.cnki.cjcmm.20180208.003.
The type 2 diabetes rat model was induced with high-sugar and high-fat diet combined with low-dose STZ. This study investigated the hypolipidemic mechanism of Coptis chinensis and C. deltoidea. After 30 days of administration, HOMA-IR and the content of TG in serum were detected, and the expressions of SCAP, SREBP-1c were tested by the method of Western blot and Real-time PCR analysis. The test results showed that both components can significantly alleviate insulin resistance and down-regulate the expressions of SREBP-1c and SCAP in liver tissue of type two diabetes mellitus. Compared with the control group, there were significant differences in relevant protein expression (<0.05, <0.01). This indicates that the inhibition of SREBP-1c and SCAP expressions may be the hypolipidemic mechanism of Coptidis Rhizoma on type 2 diabetes mellitus. The results also showed that C. deltoidea has a better efficacy in lipid elimination, but a weaker hypoglycemic effect against C. chinensis.
采用高糖高脂饮食联合低剂量链脲佐菌素诱导建立2型糖尿病大鼠模型。本研究探讨了黄连和三角叶黄连的降血脂机制。给药30天后，检测血清中HOMA-IR和TG含量，并采用蛋白质免疫印迹法和实时荧光定量PCR分析法检测SCAP、SREBP-1c的表达。试验结果表明，两种成分均能显著减轻胰岛素抵抗，并下调2型糖尿病大鼠肝组织中SREBP-1c和SCAP的表达。与对照组相比，相关蛋白表达差异有统计学意义（<0.05，<0.01）。这表明抑制SREBP-1c和SCAP的表达可能是黄连治疗2型糖尿病的降血脂机制。结果还显示，三角叶黄连在降脂方面疗效较好，但降糖作用弱于黄连。
