Chang Hong, Meng Hong-Yu, Wang Yu, Teng Zhan, Liu Shu-Min
Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
School of Pharmacy, Baotou Medical College, Baotou 014060, China.
Zhongguo Zhong Yao Za Zhi. 2018 May;43(10):2140-2146. doi: 10.19540/j.cnki.cjcmm.20180208.002.
Urinary metabolomics combined with histological progression were utilized to evaluate the therapeutic effect of Scutellariae Radix decoction and baicalin on hepatic fibrosis (HF) and explore their mechanisms, intervention targets and metabolic pathways. HF rat model was established through subcutaneous injection of CCl₄ for 8 weeks. Meanwhile, different doses of Scutellariae Radix decoction and baicalin were administered. Histomorphology of liver tissue was observed and scored by HE and Masson. Urinary metabonomic analysis based on UPLC-Q-TOF-MS was made for the changes of urinary potential biomarkers among different groups at different time points of HF. Finally, it was found that Scutellariae Radix decoction could improve HF by regulating L-tryptophan, 3-methyldioxyindole, 5-hydroxyindoleacetylglycine, kynurenic acid, 4-(2-amino-3-hydroxyphenyl)-2,4-dioxobutanoic acid, methylmalonic acid and L-leucine. However, baicalin could improve HF by regulating L-tryptophan, 3-methyldioxyindole, 5-hydroxyindoleacetylglycine, 4-(2-amino-3-hydroxyphenyl)-2,4-dioxobutanoic acid, kynurenic acid, and methylmalonic acid. These metabolites involved in tryptophan metabolism and valine, leucine and isoleucine degradation pathways. These results indicated that Scutellariae Radix had the multi-target and multi-pathway characteristics in the treatment of HF. Additionally, low-dose Scutellariae Radix decoction and baicalin are showed better efficacies, with no statistically significant difference between them in histomorphology.
采用尿液代谢组学结合组织学进展来评估黄芩汤和黄芩苷对肝纤维化（HF）的治疗效果，并探索其作用机制、干预靶点和代谢途径。通过皮下注射四氯化碳8周建立HF大鼠模型。同时，给予不同剂量的黄芩汤和黄芩苷。采用苏木精-伊红（HE）染色和Masson染色观察并评分肝组织的组织形态学。基于超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF-MS）对HF不同时间点不同组间尿液潜在生物标志物的变化进行尿液代谢组学分析。最终发现，黄芩汤可通过调节L-色氨酸、3-甲基二氧吲哚、5-羟基吲哚乙酰甘氨酸、犬尿氨酸、4-(2-氨基-3-羟基苯基)-2,4-二氧代丁酸、甲基丙二酸和L-亮氨酸来改善HF。然而，黄芩苷可通过调节L-色氨酸、3-甲基二氧吲哚、5-羟基吲哚乙酰甘氨酸、4-(2-氨基-3-羟基苯基)-2,4-二氧代丁酸、犬尿氨酸和甲基丙二酸来改善HF。这些代谢物涉及色氨酸代谢以及缬氨酸、亮氨酸和异亮氨酸降解途径。这些结果表明，黄芩在治疗HF方面具有多靶点、多途径的特点。此外，低剂量黄芩汤和黄芩苷显示出更好的疗效，它们在组织形态学上无统计学显著差异。
