利用光实现蛋白质活性的可逆、空间和时间控制。
Reversible, Spatial and Temporal Control over Protein Activity Using Light.
机构信息
University Medical Center Groningen, Department of Radiology, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands; Centre for Systems Chemistry, Stratingh Institute for Chemistry, Faculty of Science and Engineering, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands.
University Medical Center Groningen, Department of Radiology, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands; Centre for Systems Chemistry, Stratingh Institute for Chemistry, Faculty of Science and Engineering, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands.
出版信息
Trends Biochem Sci. 2018 Aug;43(8):567-575. doi: 10.1016/j.tibs.2018.05.004. Epub 2018 Jun 19.
In biomedical sciences, the function of a protein of interest is investigated by altering its net activity and assessing the consequences for the cell or organism. To change the activity of a protein, a wide variety of chemical and genetic tools have been developed. The drawback of most of these tools is that they do not allow for reversible, spatial and temporal control. Here, we describe selected developments in photopharmacology that aim at establishing such control over protein activity through bioactive molecules with photo-controlled potency. We also discuss why such control is desired and what challenges still need to be overcome for photopharmacology to reach its maturity as a chemical biology research tool.
在生物医学科学中，通过改变感兴趣的蛋白质的净活性并评估其对细胞或生物体的影响来研究其功能。为了改变蛋白质的活性，已经开发出了各种各样的化学和遗传工具。这些工具的缺点是它们不允许进行可逆的、空间和时间上的控制。在这里，我们描述了光药理学中的一些选定进展，这些进展旨在通过具有光控效力的生物活性分子来建立对蛋白质活性的这种控制。我们还讨论了为什么需要这种控制，以及为了使光药理学作为一种化学生物学研究工具达到成熟还需要克服哪些挑战。
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