Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland, PO Box 156 Archerfield, QLD 4108, Australia; CSIRO Agriculture and Food, 306 Carmody Road, St Lucia, QLD 4067, Australia.
CSIRO Agriculture and Food, 306 Carmody Road, St Lucia, QLD 4067, Australia.
Food Chem. 2018 Nov 30;267:119-123. doi: 10.1016/j.foodchem.2017.08.029. Epub 2017 Aug 8.
Indospicine, a non-proteinogenic analogue of arginine, occurs only in Indigofera plant species and accumulates in the tissues of animals grazing on Indigofera. Canine deaths have resulted from the consumption of indospicine-contaminated meat but only limited information is available regarding indospicine toxicity in humans. In this study three human cell lines, Caco-2 (colorectal adenocarcinoma), HT29-MTX-E12 (colorectal adenocarcinoma) and HepG2 (hepatocellular carcinoma), were used to investigate the cytotoxicity of indospicine and its metabolite 2-aminopimelic acid in comparison to arginine. Indospicine and 2-aminopimelic acid were more cytotoxic than arginine, displaying the highest toxicity in HepG2 liver cells. Intestinal transport in vitro also revealed a 2-fold higher transport rate of indospicine compared to arginine. The sensitivity of HepG2 cells to indospicine is consistent with observed canine hepatotoxicity, and considering the higher in vitro transport of indospicine across an intestinal barrier, it is possible that similar ill effects could be seen in humans consuming contaminated meat.
精胺酸非蛋白类似物精脒，仅存在于菘蓝属植物，会在以菘蓝属植物为食的动物组织中累积。犬类因食用受精脒污染的肉而死亡，但目前有关人类精脒毒性的资料有限。在这项研究中，我们使用三种人类细胞系，即 Caco-2（结肠直肠腺癌）、HT29-MTX-E12（结肠直肠腺癌）和 HepG2（肝细胞癌），来研究精脒及其代谢物 2-氨基庚二酸与精氨酸相比的细胞毒性。精脒和 2-氨基庚二酸比精氨酸具有更高的细胞毒性，在 HepG2 肝细胞中表现出最高的毒性。体外肠内转运也显示出精脒的转运速率比精氨酸高 2 倍。HepG2 细胞对精脒的敏感性与观察到的犬类肝毒性一致，并且考虑到精脒在穿过肠屏障时的体外转运较高，食用受污染肉类的人类可能会出现类似的不良影响。
