Nakata S, Chiba S, Terashima H, Yokoyama T, Nakao T
J Infect Dis. 1985 Aug;152(2):274-9. doi: 10.1093/infdis/152.2.274.
To assess the serum immune response in infants with gastroenteritis caused by human calicivirus (HCV), we tested a total of 165 serum specimens for antibodies to HCV by a radioimmunoassay blocking (RIA-BL) test. The specificity of the RIA-BL test for antibody to HCV was established as follows: paired sera from patients with HCV gastroenteritis revealed significant rises in antibody to HCV, whereas there was no significant change in RIA-BL titer to HCV in paired sera collected from the same individuals when infected with other gastroenteritis viruses. We analyzed 41 paired sera from patients and from healthy contacts involved in an outbreak of HCV gastroenteritis. All 23 infants without preexisting antibody revealed a sharp rise in HCV titer, and 18 (73%) of 23 of them developed illness. Fifteen of 18 infants with preexisting antibody also revealed a significant rise in HCV antibody, but only 3 (16.7%) of 18 became ill (P less than .01). These data suggest that the presence of serum antibody may correlate with resistance to illness in HCV gastroenteritis.
为评估人杯状病毒（HCV）引起的胃肠炎婴儿的血清免疫反应，我们通过放射免疫分析阻断（RIA-BL）试验对总共165份血清标本进行了HCV抗体检测。RIA-BL试验对HCV抗体的特异性确定如下：HCV胃肠炎患者的配对血清显示HCV抗体显著升高，而感染其他胃肠炎病毒的同一患者的配对血清中，RIA-BL试验检测的HCV滴度无显著变化。我们分析了41份来自HCV胃肠炎暴发中患者及健康接触者的配对血清。所有23名无预先存在抗体的婴儿HCV滴度急剧升高，其中23名中有18名（73%）发病。18名有预先存在抗体的婴儿中，15名HCV抗体也显著升高，但18名中只有3名（16.7%）发病（P<0.01）。这些数据表明，血清抗体的存在可能与HCV胃肠炎的抗病能力相关。
