Newman S L, Becker S, Halme J
J Leukoc Biol. 1985 Aug;38(2):267-78. doi: 10.1002/jlb.38.2.267.
Human peritoneal macrophages (HPM) obtained via laparoscopy were examined for the presence and functional capacity of complement and Fc receptors. Between 5 and 20 ml of peritoneal fluid containing 1-2 X 10(6) macrophages/ml was available for each study. Macrophages made up 80-95% of the cells in the fluid. Fc and C3 receptors on HPM were characterized by rosette formation with, and phagocytosis of, IgG- and C3-coated sheep erythrocytes (E). ElgG were bound by 82% and ingested by 63% of HPM, with 4-15 E ingested/HPM. The HPM formed rosettes with EC3b (56%) and EC3bi (71%) but not EC3d,g or EC3d. Antibodies to complement receptors type 1 (CR1) and type 3 (CR3) inhibited rosette formation with EC3b and EC3bi, respectively, indicating that HPM possessed separate and distinct receptors for the C3b and iC3b ligands. In 60% of the samples studied, HPM demonstrated the ability to ingest both EC3b and EC3bi, as well as ElgG. Because of the heterogeneous nature of the cells obtained in peritoneal fluid, due to their progressive change from monocytelike cells into mature macrophages, HPM were separated by 1 g velocity sedimentation into fractions of increasing maturity. They were then examined for phagocytosis via Fc and complement receptors. Fc receptor mediated phagocytosis occurred throughout the monocyte-to-macrophage maturation sequence, while the ability of HPM to ingest via CR1 and CR3 was maturation dependent, with ingestion via CR3 occurring before CR1, in a manner analogous to in vitro differentiation of monocyte-derived macrophages.
通过腹腔镜检查获取的人腹膜巨噬细胞（HPM）被检测补体和Fc受体的存在情况及功能能力。每次研究可获得5至20毫升含有1 - 2×10⁶个巨噬细胞/毫升的腹膜液。巨噬细胞占液体中细胞的80%至95%。HPM上的Fc和C3受体通过与IgG包被和C3包被的绵羊红细胞（E）形成玫瑰花结以及对其进行吞噬来表征。82%的HPM能结合IgG，63%能摄取IgG，每个HPM摄取4至15个E。HPM与EC3b（56%）和EC3bi（71%）形成玫瑰花结，但不与EC3d、g或EC3d形成玫瑰花结。针对补体受体1型（CR1）和3型（CR3）的抗体分别抑制与EC3b和EC3bi的玫瑰花结形成，表明HPM对C3b和iC3b配体具有单独且不同的受体。在60%的研究样本中，HPM表现出摄取EC3b和EC3bi以及IgG的能力。由于腹膜液中获得的细胞具有异质性，因其从单核样细胞逐渐转变为成熟巨噬细胞，HPM通过1g速度沉降被分离成成熟度增加的组分。然后通过Fc和补体受体检测它们的吞噬作用。Fc受体介导的吞噬作用在单核细胞到巨噬细胞的整个成熟序列中都存在，而HPM通过CR1和CR3摄取的能力取决于成熟度，通过CR3摄取发生在CR1之前，其方式类似于单核细胞衍生巨噬细胞的体外分化。
