Martínez-Hernández J M, Ballados-González G G, Fernández-Bandala D, Martínez-Soto S, Velázquez-Osorio V, Martínez-Rodríguez P B, Cruz-Romero A, Grostieta E, Lozano-Sardaneta Y, Colunga Salas P, Becker I, Sánchez-Montes S
Módulo de Ovinos y Caprinos, Posta Zootécnica Torreón del Molino, Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Xalapa, Mexico.
Facultad de Medicina Veterinaria y Zootecnia, región Veracruz, Universidad Veracruzana, Veracruz, Mexico.
Trop Anim Health Prod. 2019 Jan;51(1):243-248. doi: 10.1007/s11250-018-1648-x. Epub 2018 Jun 22.
Mycoplasma ovis is a small, pleiotropic bacterium, which parasitizes the external surface of erythrocytes of several species of artiodactyl mammals, especially sheep and goats. We here report an outbreak of ovine mycoplasmosis in a sheep flock of a private ranch (Universidad Veracruzana) in Veracruz, Mexico. For the identification of Mycoplasma and other hemoparasitic bacterial agents, we stained blood smears with the DiffQuick® technique and additionally amplified several fragments of 16S rDNA gene. We detected the presence of morulas in erythrocytes from 30 sick female adult sheep, and found Mycoplasma ovis DNA in all of them. Furthermore, three of these animals also tested positive for Anaplasma ovis. Our findings represent the first record of M. ovis and A. ovis in an outbreak of hemolytic anemia in a sheep flock, leading to severe livestock loss in a ranch of Mexico. This study highlights the importance of establishing an active surveillance of both pathogens in the country.
绵羊支原体是一种小型的、具有多效性的细菌，它寄生于多种偶蹄目哺乳动物（尤其是绵羊和山羊）的红细胞外表面。我们在此报告了墨西哥韦拉克鲁斯州一个私人牧场（韦拉克鲁斯大学）的一群绵羊中发生的绵羊支原体病疫情。为了鉴定支原体和其他血液寄生细菌病原体，我们用DiffQuick®技术对血涂片进行染色，并额外扩增了16S rDNA基因的几个片段。我们在30只患病成年母羊的红细胞中检测到桑葚体的存在，并在所有这些羊中都发现了绵羊支原体DNA。此外，其中三只动物的绵羊无形体检测也呈阳性。我们的研究结果代表了在一群绵羊的溶血性贫血疫情中首次记录到绵羊支原体和绵羊无形体，导致墨西哥一个牧场的牲畜严重损失。这项研究强调了在该国对这两种病原体建立主动监测的重要性。
