Department of Chemistry, Faculty of Sciences, Allama Iqbal Open University, Islamabad, 44000, Pakistan.
Appl Biochem Biotechnol. 2018 Dec;186(4):1090-1110. doi: 10.1007/s12010-018-2818-z. Epub 2018 Jun 22.
Cancer is developed by rapid, uncontrolled, and abnormal cell proliferation and one of the leading causes of deaths worldwide in human beings. For the remedial measures of preventing different types of cancers, one of the research domains that have gained substantial importance in medical science is the development of new metallo-drugs and their investigations as potential anticancer drug agents by using various analytical techniques. Since metal-based complexes show weak absorption bands, electrochemical methods are considered more feasible and preferable over spectroscopic methods for easy characterization. Due to closer resemblance of electrochemical and biological processes, cyclic voltammetry among different electrochemical methods is considered the most versatile for the study of in-vitro metal-based drug-DNA interactions in terms of changes in the redox activities. Current potential data of a metal complex leads to determine binding kinetics in terms of binding constant and binding site size that involve determining the binding mode of drug with DNA, i.e., electrostatic interactions, intercalation, or minor-major groove binding. Binding parameters and modes of interactions, further, help to develop the mechanism of action of drug with the DNA. In this review, we emphasize on cyclic voltammetric DNA binding studies on some metal complexes that have been carried out in the last three decades for the investigation of their anticancer potentials.
癌症是由快速、不受控制和异常的细胞增殖引起的，是全球人类死亡的主要原因之一。为了预防不同类型癌症的治疗措施，医学科学中一个备受关注的研究领域是开发新型金属药物，并利用各种分析技术将其作为潜在的抗癌药物进行研究。由于基于金属的配合物显示出较弱的吸收带，电化学方法比光谱方法更可行和可取，因为电化学方法更易于进行特征描述。由于电化学和生物过程更相似，在不同的电化学方法中，循环伏安法被认为是研究体外金属基药物-DNA 相互作用的最通用方法，因为它可以根据氧化还原活性的变化来研究。金属配合物的电流电位数据可用于确定结合动力学，包括结合常数和结合位点大小，这涉及确定药物与 DNA 的结合模式，即静电相互作用、嵌入或小沟-大沟结合。结合参数和相互作用模式进一步有助于阐明药物与 DNA 的作用机制。在这篇综述中，我们强调了过去三十年中进行的一些金属配合物的循环伏安法 DNA 结合研究，以研究它们的抗癌潜力。
