Robbins S J, Eagle J P
J Gen Virol. 1985 Sep;66 ( Pt 9):2065-70. doi: 10.1099/0022-1317-66-9-2065.
The intracellular distribution of measles virus inclusion bodies in persistently infected human cells (AV3A1/MV) changed markedly following continuous exposure to 3', 5' cyclic adenosine monophosphate (cAMP). When assayed by immunofluorescence, the number of cells with intranuclear virus inclusions increased from 5 to 10% to 80 to 90% after exposure to 1 mM-cAMP for 4 days. Exposure of cells to cAMP also resulted in a twofold increase in the average number of inclusions in invaded nuclei. Similar but less pronounced changes occurred in cells treated with inducers of adenylate cyclase and an inhibitor of phosphodiesterase. Examination of cAMP-treated cells by electron microscopy indicated that viral inclusion bodies consisted of typical helical nucleocapsids. No evidence of nucleocapsids crossing the nuclear membrane (through nuclear pores) was found.
在持续感染的人细胞（AV3A1/MV）中，麻疹病毒包涵体的细胞内分布在持续暴露于3'，5'-环磷酸腺苷（cAMP）后发生了显著变化。通过免疫荧光检测，暴露于1 mM cAMP 4天后，有核内病毒包涵体的细胞数量从5%至10%增加到80%至90%。细胞暴露于cAMP还导致侵入细胞核中包涵体的平均数量增加了两倍。用腺苷酸环化酶诱导剂和磷酸二酯酶抑制剂处理的细胞也出现了类似但不太明显的变化。通过电子显微镜检查cAMP处理的细胞表明，病毒包涵体由典型的螺旋核衣壳组成。未发现核衣壳穿过核膜（通过核孔）的证据。
