Kinane K A, Hillary I B
J Med Virol. 1985 Aug;16(4):375-84. doi: 10.1002/jmv.1890160411.
An indirect ELISA and an inhibition ELISA were developed for the detection of cytomegalovirus (CMV)-specific immunoglobulin G (IgG) and CMV-specific total immunoglobulin, respectively. Both assays were more specific than the complement fixation (CF) test, and titres of positive sera were 660 times higher by IgG ELISA and 6 times higher by inhibition ELISA than titres by the CF test. Titres by IgG ELISA were reliably determined using the absorbance obtained at a single serum dilution of 1/1,000 in conjunction with a standard graph. Both ELISAs compared favourably with each other in sensitivity and specificity in determining CMV immune status. The inhibition ELISA, in particular, provides a simple and reliable method of screening sera, which requires no control antigen or predilution of sera. It should prove useful for large-scale screening procedures, such as blood donor testing.
分别开发了一种间接酶联免疫吸附测定法（ELISA）和一种抑制酶联免疫吸附测定法，用于检测巨细胞病毒（CMV）特异性免疫球蛋白G（IgG）和CMV特异性总免疫球蛋白。这两种测定法均比补体结合（CF）试验更具特异性，IgG ELISA检测阳性血清的效价比CF试验高660倍，抑制ELISA检测阳性血清的效价比CF试验高6倍。通过在1/1000单一血清稀释度下获得的吸光度结合标准曲线，可可靠地测定IgG ELISA的效价。在确定CMV免疫状态方面，两种ELISA在敏感性和特异性上相互比较效果良好。特别是抑制ELISA提供了一种简单可靠的血清筛查方法，无需对照抗原或血清预稀释。它对于大规模筛查程序，如献血者检测，应是有用的。
