酶联免疫吸附测定法用于在单一血清稀释度下检测抗巨细胞病毒和风疹病毒抗体。
Enzyme-linked immunosorbent assay for measurement of antibody against cytomegalovirus and rubella virus in a single serum dilution.
作者信息
van Loon A M, van der Logt J T, van der Veen J
出版信息
J Clin Pathol. 1981 Jun;34(6):665-9. doi: 10.1136/jcp.34.6.665.
Abstract
Enzyme-linked immunosorbent assays (ELISA) were developed for quantifying cytomegalovirus (CMV) and rubella antibodies using a single serum dilution (1/800) in conjunction with a standard curve. A near linear relation was found between the logarithms of absorbance values of sera at a dilution of 1/800 and the titres as determined by an end point dilution ELISA. The reproducibility of the single dilution ELISA was good; the within-test coefficients of variation averaged 7.5% for CMV antibody and 12.4% for rubella antibody. A close correlation was found between ELISA and complement-fixing (CF) antibody titres to CMV and between ELISA and haemagglutination-inhibition (HI) antibody titres to rubella virus. The titres in ELISA were 200 to 1000 times higher than in CF for CMV and 50 to 100 times higher than in HI for rubella virus.
摘要
酶联免疫吸附测定（ELISA）被开发用于使用单一血清稀释度（1/800）结合标准曲线来定量巨细胞病毒（CMV）和风疹抗体。在血清1/800稀释度下吸光度值的对数与终点稀释ELISA测定的滴度之间发现了近似线性关系。单稀释ELISA的重现性良好；CMV抗体的试验内变异系数平均为7.5%，风疹抗体的试验内变异系数平均为12.4%。在ELISA与CMV的补体结合（CF）抗体滴度之间以及ELISA与风疹病毒的血凝抑制（HI）抗体滴度之间发现了密切相关性。对于CMV，ELISA中的滴度比CF中的滴度高200至1000倍，对于风疹病毒，ELISA中的滴度比HI中的滴度高50至100倍。
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2
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